FastQCFastQC Report
Thu 22 Jun 2023
ERR2382520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569309
Total Bases77.3 Mbp
Sequences flagged as poor quality0
Sequence length35-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA44387628.284805605524472Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA21841413.917845370159732Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA18158811.571207455000895Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA437392.787150268047912Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA299471.9082921209271086Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA259951.6564615381674352Illumina PCR Primer Index 6 (100% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC131430.8375023656908868No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA91190.5810837763627177Illumina PCR Primer Index 6 (96% over 29bp)
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATATCTCG86480.5510705667271392No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATATCTC78000.4970340449204076No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA72420.4614769940145631Illumina PCR Primer Index 6 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCGTCG64200.4090972523575663Illumina Single End PCR Primer 1 (100% over 33bp)
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATATCT63040.4017054639972115No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG62540.39851934832464475No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT61970.39488717645791876No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATATCTCGT58090.37016291883880104No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATATC44990.28668668821755305No Hit
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATATCTCGT42700.2720942784371975No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA42200.2689081627646308Illumina PCR Primer Index 6 (96% over 29bp)
AAAACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATATC34930.22258204088551076No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG30600.194990279161083No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC30470.19416188908621565No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATATCTCGT29940.19078460647329493No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTC28760.1832653734860375No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATATCTCG27660.1762559190063907No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA27580.17574614049878004Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT25340.16147234228568114No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24460.15586477870196372Illumina PCR Primer Index 6 (96% over 29bp)
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATATCTC24380.15535500019435305No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC24370.15529127788090175No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA24020.15306099691010502Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA23780.151531661387273Illumina PCR Primer Index 6 (96% over 29bp)
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATATCTC23090.14713482175913092No Hit
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA22610.14407615071346688Illumina PCR Primer Index 6 (100% over 28bp)
CCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATATCTCG22030.14038025653328948No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT22010.14025281190638683No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAA21840.13916953257771414Illumina PCR Primer Index 6 (96% over 28bp)
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATATCTCGT21170.13490013757647476No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT20980.1336894136208994No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATATC20870.13298846817293472No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA19960.1271897376488633Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC19250.12266545339381855No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATATCT18740.11941561540780049No Hit
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA18440.11750394600426048Illumina PCR Primer Index 6 (100% over 28bp)
AAACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATATCT17840.11368060719718041No Hit
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA17750.1131071063761184Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT17420.11100427003222436No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT17400.1108768254053217No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA17200.10960237913629502Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA16930.10788187667310899Illumina PCR Primer Index 6 (100% over 28bp)
AAATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATATCTCG16750.10673487503098497No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTC16740.10667115271753365No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAA16110.10265664697009957Illumina PCR Primer Index 6 (96% over 27bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC15710.10010775443204621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)