FastQCFastQC Report
Thu 22 Jun 2023
ERR2382523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3809496
Total Bases510.8 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA60881715.981562915409283Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG2669877.008459911757356No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA1170043.071377421055174No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1154923.0316871313160587Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1065152.796039161085876Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT954952.5067620493629605No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA844072.2156999246094498Illumina PCR Primer Index 6 (96% over 29bp)
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG720881.892323813963842No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG502441.318914628076785No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA464701.2198464048787556Illumina PCR Primer Index 6 (100% over 28bp)
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG439191.152882166039812No Hit
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG384841.0102123745503342No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC370750.9732258545487381No Hit
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT258890.6795912110158404No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA257550.6760736853379029Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT207420.5444814747147655No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTGT192060.5041611803766168No Hit
ACGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG167670.44013696299982985No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA152250.3996591675119229Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT151910.3987666609966253No Hit
TTTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT145380.38162528586458677No Hit
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG139170.3653239168645931No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG133910.35151631606910727No Hit
ACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTC133560.35059755936218334No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA132690.34831379269068663Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA132580.34802504058279626Illumina PCR Primer Index 6 (100% over 28bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGCAAGCTGT130470.3424862501496261No Hit
TTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAA126280.33148741985816493No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT125920.3305424129596146No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA122950.32274610604657417Illumina PCR Primer Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC118350.31067101789843066No Hit
TTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA117840.3093322581254843No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA113350.29754592208523123No Hit
TTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGA112490.2952884056053609No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG112260.29468465119795373No Hit
TCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA109380.28712459600955087Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC106880.28056204810295116No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA106200.278777035072356No Hit
TTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAG95680.2511618334813844No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT94300.24753930703694135No Hit
CAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA94300.24753930703694135No Hit
AAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACCAT93180.2445992855747847No Hit
TTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTG90060.23640922578734824No Hit
AAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAC88740.2329442004926636No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG88530.23239294646850922No Hit
TCATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT88220.23157919052809087No Hit
AAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACT86740.2276941621673838No Hit
ACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACCA85190.22362538246529198No Hit
TAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAG84750.22247037403373046No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC84430.2216303679016857No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC83390.21890034797254018No Hit
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTG83180.2183490939483858No Hit
TCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGG82490.2165378307261643No Hit
GGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG81310.21344030811424924No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC80390.21102529048462054No Hit
ATTTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGG79270.20808526902246385No Hit
TAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCAC75510.1982151969709379No Hit
TTATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT74820.19640393374871634No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA74620.19587892991618838Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA72220.18957888392585268Illumina PCR Primer Index 6 (96% over 29bp)
TTAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGT71170.18682261380508078No Hit
TTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTG70400.18480134904984807No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA70050.1838825923429241No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA69190.18162507586305382Illumina PCR Primer Index 6 (96% over 29bp)
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG67380.1768737911786756No Hit
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACCAT64760.1699962409725591No Hit
TTGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA61190.16062492256193472No Hit
GCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTA60720.15939116355549396No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC60020.15755365014164605No Hit
ACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAG59870.15715989726725005No Hit
TTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCA55580.14589856505952495No Hit
TATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTC55010.1444023041368202No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAA54260.14243353976484027No Hit
AACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACC53850.14135728190815794No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA53650.14083227807562995No Hit
TTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA53520.14049102558448678No Hit
CTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG52180.1369734999065493No Hit
AGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG51600.13545098879221817No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC51250.13453223208529422No Hit
TCATTTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGC50790.13332472327047987No Hit
TCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTT50180.13172346158126955No Hit
TCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTA50110.13153971023988476No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC49980.13119845774874156No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT49120.12894094126887126No Hit
TAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGT48470.12723467881315534No Hit
TTTTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGG47880.12568591750719782No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA47470.12460965965051544Illumina PCR Primer Index 6 (96% over 29bp)
TTGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG47450.12455715926726264No Hit
TCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCAT46950.1232446496859427No Hit
TAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCG46930.1231921493026899No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC45450.11930712094198287No Hit
TCGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTC44240.1161308477551886No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA44180.11597334660543021No Hit
TTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAACAAGGTTTC44130.11584209564729822No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC44090.11573709488079263No Hit
TAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTG43900.11523834123989105No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC42600.1118258163284592No Hit
TTTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG41600.1092007971658193No Hit
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGCAAGCTG41360.10857079256678574No Hit
ATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATCTCGTATGCC40900.10736328375197139No Hit
ACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATT40760.10699578106920181No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT40460.10620827532040984No Hit
TAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGA40220.10557827072137627No Hit
TCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAAC40170.10544701976324428No Hit
TTATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT40070.10518451784698028No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA39990.10497451631396909Illumina PCR Primer Index 6 (96% over 29bp)
TTGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGG38890.1020869952350652No Hit
TGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGC38220.10032823239609649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)