FastQCFastQC Report
Thu 22 Jun 2023
ERR2382525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024924
Total Bases271.5 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA61206630.226615912498445Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC1923489.49902317321539No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1376686.798674913231312Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA917074.528910714673736Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA816104.030274716483186Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA594172.934282965681675Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC487072.4053742263907187No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA427502.1111903459092787Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT279161.3786196420211327No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT265351.3104195515486015No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC189910.9378623592786692No Hit
TGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCC175120.8648225809956325No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT151520.7482749969875413No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA141820.700371964577436Illumina PCR Primer Index 6 (100% over 28bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA128570.6349374099966221Illumina PCR Primer Index 6 (96% over 29bp)
CTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC123690.6108377400830846No Hit
GTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC119310.5892072986442948No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA109710.5417981119291391Illumina PCR Primer Index 6 (96% over 29bp)
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC97280.4804130920469114No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC79110.3906813292745802Illumina PCR Primer Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC78400.3871750248404385No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA65620.3240615450258874RNA PCR Primer, Index 37 (96% over 27bp)
ATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA57620.28455388942992427Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA57410.28351681347053026TruSeq Adapter, Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATAGCTCGTATGC57200.2824797375111362No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA53290.2631703708386092Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA53240.2629234479911345No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTGT51250.25309591866163866No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA49280.24336715847113277Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA44630.2204033336559792Illumina PCR Primer Index 6 (96% over 29bp)
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG41540.20514350168203843No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA37150.18346367567375368No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA36560.1805499860735514Illumina PCR Primer Index 6 (96% over 29bp)
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT35330.1744756840256721No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG35070.1731916852188033No Hit
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA32810.1620307725129437Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA31200.15407985682425612Illumina PCR Primer Index 6 (96% over 30bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA30130.14879570788829605Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30120.1487463233188011Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA29190.1441535583557704Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA27660.13659771922304245Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC27590.13625202723657776No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG26710.1319061851210218No Hit
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA26170.1292394183682943RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA25650.1266714207545567Illumina PCR Primer Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA24670.12183173294405124Illumina PCR Primer Index 6 (96% over 29bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA24530.12114034897112189Illumina PCR Primer Index 6 (96% over 29bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC24510.12104157983213197No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC24480.1208934261236471No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA24230.11965881188627325No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC23350.11531296977071732No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC23010.11363389440788889No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA22720.11220174189253522No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG22210.10968312884829258No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATATCGTATGC20810.10276928911899903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)