FastQCFastQC Report
Thu 22 Jun 2023
ERR2382527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096873
Total Bases103.8 Mbp
Sequences flagged as poor quality0
Sequence length35-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA31698115.116843032458332Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1016124.845882416340904Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA787393.755067665042184Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA582612.778470608377331Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA523282.4955254800839155Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA248331.1842872696629696Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT205810.981509132885015No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA197140.9401618505269512Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA188080.8969546558136806Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA122330.5833925087499339Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT104370.4977411602896313No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT91030.43412261972947336No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT87620.41786030913650946No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA85520.40784539645462553Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA76880.3666411842777316Illumina PCR Primer Index 6 (100% over 28bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT70990.3385517387080667No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT65360.3117022347085398No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGT61060.29119550874087274No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA54580.2602923496082023Illumina PCR Primer Index 6 (100% over 28bp)
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT38120.1817945102063883No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTAT37760.18007766803235103No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT37230.17755009483168507No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGT32670.15580342729387997No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT31380.14965140950357986No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30700.14640848539706505Illumina PCR Primer Index 6 (96% over 29bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGT30390.14493009352497743No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG30170.14388091219639912No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA29190.13920728627818663Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA27450.13090921577033993Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA27100.1292400636566926Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC25020.11932053109558853No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT22810.10878102774941545No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT21900.1044412322539324No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG21080.10053064730195867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)