FastQCFastQC Report
Thu 22 Jun 2023
ERR2382529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098735
Total Bases281.3 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24416611.633960457132511Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC1148565.472629941369444No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1078545.139000397858711Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA961884.583141749673017Illumina PCR Primer Index 6 (100% over 28bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT659873.1441320605031122No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT554562.642353608244967No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA451322.150438240178012Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT397681.8948557107019228No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA377091.7967489940368842No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA377001.7963201642894409Illumina PCR Primer Index 6 (96% over 29bp)
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG318111.5157225662125042No Hit
CGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTG288201.3732081468122463No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT269191.28262977460232No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA241911.1526467133773441Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA211751.0089411002341886Illumina PCR Primer Index 6 (100% over 28bp)
GGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTG211191.0062728262500982No Hit
TCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACT135650.6463417248961875No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA134650.6415769499245975No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC133540.6362880497061325No Hit
TTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG117120.5580504446726242No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG106970.5096879787109854No Hit
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG97390.46404143448315294No Hit
TGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAATG96490.4597531370087219No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT93320.4446488003487815No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA92470.44059874162293Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA84510.40267113284907335Illumina PCR Primer Index 6 (96% over 29bp)
GATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG78710.3750354380138512No Hit
AATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG77840.37089008378856786No Hit
GCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACT76880.36631589981584145No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG75220.358406373363002No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC67990.3239570503184061No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA61510.2930813085025027Illumina PCR Primer Index 6 (96% over 29bp)
GGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAATG58980.2810264278243799No Hit
TGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTG55780.26577914791529184No Hit
CATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG55420.26406382892551944No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCT47270.22523091290706068No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG45180.21527253321643752No Hit
GGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC44760.21327132772836968No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG44560.2123183727340517No Hit
TCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAG44470.2118895429866086No Hit
CCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACT41890.1995964235599063No Hit
ACCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTC39920.19020981686587396No Hit
ATATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCA39570.18854214562581748No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA39030.18596916714115883Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTA38690.18434914365081823No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA37200.17724962894314908Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTC36010.17157954672695697No Hit
CCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAA35580.16953069348917324No Hit
CTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG35010.16681477175536694No Hit
GTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG33460.15942937054940237No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA32540.15504577757553956Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTCT32050.15271103783946044Illumina PCR Primer Index 6 (95% over 21bp)
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAA31640.1507574801011085No Hit
TTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGA30830.1468980123741206No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG29520.14065615716133767No Hit
ATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTA29290.13956025891787197No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA27460.13084072071986222Illumina PCR Primer Index 6 (100% over 28bp)
GGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGC27230.1297448224763965No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC26750.12745773049003328No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC26110.12440827450821566No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC25640.12216883027156836No Hit
GGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG25260.12035821578236415No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG24440.11645110030566032No Hit
TTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA24420.11635580480622851No Hit
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA24170.11516461106333101TruSeq Adapter, Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC24070.114688133566172No Hit
TTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTG23970.11421165606901301No Hit
TTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT23540.11216280283122927No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22750.10839863060367316Illumina PCR Primer Index 6 (96% over 29bp)
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG22460.10701684586191205No Hit
TGATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCA22410.10677860711333256No Hit
TTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG21370.10182324114287893No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA21300.10148970689486762Illumina PCR Primer Index 6 (96% over 29bp)
TTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAG21050.10029851315197012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)