FastQCFastQC Report
Thu 22 Jun 2023
ERR2382533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211786
Total Bases163.5 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA62261751.38011166988231Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA17146514.149775620447835Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA915417.554221619989009Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA410413.386819124828971Illumina PCR Primer Index 6 (100% over 28bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC215491.7782842845188835No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA155511.2833124000442322Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA139421.1505331799509153Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA137621.1356790720473746Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA134231.1077038354957063Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA90910.7502149719504929Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA64430.5316945401250716Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA58640.4839138263686823Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT44690.36879449011624166No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA44420.36656637393071057Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA40320.3327320170393122Illumina PCR Primer Index 6 (100% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA38240.31556727012855407Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA34370.2836309381359415Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA34140.2817329132371557Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA28460.2348599505193161Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA26750.22074854801095242Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24130.19912756872913204Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24050.19846738615564136Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA23450.1935160168544611Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCTTC21450.17701145251719363No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAA19560.16141463921847587Illumina PCR Primer Index 6 (96% over 28bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA19350.1596816599630628Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAAA17840.14722071388842584Illumina PCR Primer Index 6 (96% over 27bp)
CTGTAGGCACCATCAATATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16910.13954609147159647Illumina Single End Adapter 1 (95% over 22bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16100.13286174291500316Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA14840.12246386738252464RNA PCR Primer, Index 44 (100% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA14270.11776006654640342Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACAATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA13690.11297374288859585Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA13540.11173590056330078Illumina PCR Primer Index 6 (96% over 28bp)
CTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12140.10018270552721355Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)