FastQCFastQC Report
Thu 22 Jun 2023
ERR2382537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC40309610.0774No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC1538033.845075No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1487673.7191750000000003Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1038882.5972Illumina PCR Primer Index 6 (96% over 29bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1024612.561525No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1015012.537525No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG987092.467725No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA967302.41825Illumina PCR Primer Index 6 (100% over 28bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA823862.05965No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA765461.91365Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT591061.47765No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT518871.297175No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC430821.07705No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA411291.028225Illumina PCR Primer Index 6 (96% over 29bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC277390.693475No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT259450.648625No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC242390.605975No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC234220.58555No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCT216210.540525No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA207520.5188No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG199940.49985No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC196590.491475No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT184330.46082500000000004No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA182060.45515Illumina PCR Primer Index 6 (100% over 28bp)
CAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCG180740.45185No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT177150.442875No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC167670.419175No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT160290.40072500000000005No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATATCT151750.37937499999999996No Hit
GAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC149810.374525No Hit
CGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCTC116410.29102500000000003No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT115610.289025No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT97390.243475No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGTA96530.241325No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA92870.232175No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC89540.22385No Hit
AAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC79660.19915000000000002No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC77550.193875No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA76830.192075Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA75630.189075Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA74740.18685Illumina PCR Primer Index 6 (100% over 28bp)
GCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC72830.18207500000000001No Hit
AGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCGT69420.17355No Hit
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT68160.1704No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGT65160.1629No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCGT64920.1623No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC63630.159075No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC60050.150125No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA59310.148275Illumina PCR Primer Index 6 (96% over 29bp)
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT58200.1455No Hit
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG57280.1432No Hit
ACAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT53300.13324999999999998No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA53060.13265Illumina PCR Primer Index 6 (96% over 29bp)
TATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCG51970.129925No Hit
TCGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATATCT51290.128225No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCG50820.12705No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC49390.123475No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAAA46810.117025Illumina PCR Primer Index 6 (96% over 27bp)
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG46200.1155No Hit
GGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA46070.115175No Hit
CAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT43930.109825No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATCT43110.107775No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43030.107575Illumina PCR Primer Index 6 (96% over 29bp)
CAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATATCTC42430.106075No Hit
GGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCGT41690.104225No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC41560.10389999999999999No Hit
CAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATCTCGT40980.10245No Hit
ACGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATATCT40900.10225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)