FastQCFastQC Report
Thu 22 Jun 2023
ERR2382538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC3959579.898925No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC1520673.8016750000000004No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1474133.6853249999999997Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1083262.7081500000000003Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1025502.56375Illumina PCR Primer Index 6 (100% over 28bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1009242.5231No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1005382.51345No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG984452.461125No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA800432.001075No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA743971.859925Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT581101.45275No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT512691.281725No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA439551.098875Illumina PCR Primer Index 6 (96% over 29bp)
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC427781.06945No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC278490.696225No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT258020.64505No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC238040.5951No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC232080.5801999999999999No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCT215670.539175No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA212970.532425No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC199520.4988No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG198570.496425No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA192240.4806Illumina PCR Primer Index 6 (100% over 28bp)
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT182080.45519999999999994No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT179650.44912499999999994No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCG178400.44600000000000006No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC164710.411775No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT163750.40937500000000004No Hit
GAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC150560.3764No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATATCT147780.36945No Hit
CGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCTC116500.29125No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT115710.289275No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGTA95550.23887499999999998No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA93190.232975No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT92220.23054999999999998No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC89290.22322499999999998No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA82520.2063Illumina PCR Primer Index 6 (96% over 29bp)
AAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC80050.200125No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA76020.19005Illumina PCR Primer Index 6 (96% over 29bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC75580.18894999999999998No Hit
GCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC72270.180675No Hit
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT70140.17535No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA69820.17455Illumina PCR Primer Index 6 (100% over 28bp)
AGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCGT69450.173625No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCGT67750.169375No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGT65200.163No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC60780.15195No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC59260.14815No Hit
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG58970.147425No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT58960.1474No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA53540.13385Illumina PCR Primer Index 6 (96% over 29bp)
TATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCG53280.1332No Hit
ACAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT52120.1303No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCG51580.12895No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA50680.12669999999999998Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA48530.12132499999999999Illumina PCR Primer Index 6 (96% over 29bp)
TCGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATATCT47480.11869999999999999No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC46940.11735000000000001No Hit
GGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA46540.11635000000000001No Hit
CAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT45640.11410000000000001No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG45510.11377500000000002No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATCT43810.10952500000000001No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATATCTC41830.104575No Hit
GGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCGT41090.102725No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC40580.10145No Hit
CAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATCTCGT40190.100475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)