FastQCFastQC Report
Thu 22 Jun 2023
ERR2382540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC3962579.906425No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC1515473.7886749999999996No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1490433.7260750000000002Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1034322.5858Illumina PCR Primer Index 6 (96% over 29bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1007822.5195499999999997No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1002872.507175No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA995002.4875000000000003Illumina PCR Primer Index 6 (100% over 28bp)
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG926392.315975No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA801122.0028No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA741391.853475Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT587861.46965No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT513621.28405No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC424051.060125No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA409731.0243250000000002Illumina PCR Primer Index 6 (96% over 29bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC278720.6968No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT255830.639575No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC236410.591025No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC231690.579225No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCT212840.5321No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA201270.5031749999999999No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC195910.489775No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA189010.47252500000000003Illumina PCR Primer Index 6 (100% over 28bp)
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG185080.4627No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT185000.46249999999999997No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT177810.44452500000000006No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCG168460.42115No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC164230.410575No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT156690.391725No Hit
GAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC150180.37545No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATATCT149830.374575No Hit
CGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCTC116230.29057499999999997No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT113960.2849No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT94130.23532499999999998No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGTA90720.2268No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC89110.222775No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA87040.2176No Hit
AAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC79060.19765No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA79030.197575Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA77770.19442500000000001Illumina PCR Primer Index 6 (96% over 29bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC73730.18432500000000002No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCT72150.180375No Hit
GCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC71920.1798No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA68780.17195000000000002Illumina PCR Primer Index 6 (100% over 28bp)
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT68530.171325No Hit
AGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCGT63390.158475No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCGT63010.157525No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGT60840.1521No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC60710.151775No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC60640.1516No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT59660.14915No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA54580.13645000000000002Illumina PCR Primer Index 6 (96% over 29bp)
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG54040.1351No Hit
ACAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT52920.1323No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA50910.127275Illumina PCR Primer Index 6 (96% over 29bp)
TATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCG48720.12179999999999999No Hit
TCGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATATCT47900.11975No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCG47560.11889999999999999No Hit
CAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT46400.116No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC46290.11572500000000001No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA45370.11342500000000001Illumina PCR Primer Index 6 (96% over 29bp)
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATCT43670.10917500000000001No Hit
GGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA43640.1091No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAA43520.1088Illumina PCR Primer Index 6 (96% over 28bp)
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG42620.10655No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATATCTC42170.105425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)