FastQCFastQC Report
Thu 22 Jun 2023
ERR2382542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC3908379.770925No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC1505673.7641750000000003No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1444753.611875Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1056212.6405250000000002Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1014462.5361499999999997Illumina PCR Primer Index 6 (100% over 28bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT996432.491075No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT988342.47085No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG964512.411275No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA790611.976525No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA741541.85385Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT580551.451375No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT506101.26525No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA430311.075775Illumina PCR Primer Index 6 (96% over 29bp)
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC418631.046575No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC277290.693225No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT252850.6321249999999999No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC235520.5888No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC228300.57075No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCT213790.5344749999999999No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA207210.518025No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG198160.4954No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC196130.49032499999999996No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA184780.46195Illumina PCR Primer Index 6 (100% over 28bp)
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT181880.4547No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCG175330.438325No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT174080.4352No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC161640.4041No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT158460.39615No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATATCT147710.36927499999999996No Hit
GAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC147510.368775No Hit
CGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCTC115520.2888No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT113390.283475No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGTA94910.23727499999999999No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT92150.23037499999999997No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA90790.226975No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC87880.2197No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA80050.200125Illumina PCR Primer Index 6 (96% over 29bp)
AAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC78400.196No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC75210.188025No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA74240.1856Illumina PCR Primer Index 6 (96% over 29bp)
GCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC72160.1804No Hit
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT69570.173925No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA68180.17045000000000002Illumina PCR Primer Index 6 (100% over 28bp)
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCGT67630.169075No Hit
AGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCGT66300.16574999999999998No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATATCTC65140.16285No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGT63770.15942499999999998No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC61250.153125No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC60590.151475No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT59990.149975No Hit
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG57760.1444No Hit
TATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCG53350.13337500000000002No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52410.131025Illumina PCR Primer Index 6 (96% over 29bp)
ACAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT52390.13097499999999998No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCG50590.126475No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA50480.1262Illumina PCR Primer Index 6 (96% over 29bp)
TCGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATATCT47880.11969999999999999No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC45790.114475No Hit
CAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT45400.11349999999999999No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA45240.1131Illumina PCR Primer Index 6 (96% over 29bp)
GGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA45130.112825No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG44290.11072499999999999No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATCT44120.11030000000000001No Hit
GGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCGT40240.1006No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC40120.1003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)