FastQCFastQC Report
Thu 22 Jun 2023
ERR2382543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC3980829.95205No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC1518843.7971No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1487993.719975Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1052112.630275Illumina PCR Primer Index 6 (96% over 29bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1007452.518625No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1001282.5031999999999996No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA977882.4447Illumina PCR Primer Index 6 (100% over 28bp)
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG963932.409825No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA811532.028825No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA757401.8935Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT586361.4659No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT512971.2824250000000001No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC426871.067175No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA422381.05595Illumina PCR Primer Index 6 (96% over 29bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC275850.6896249999999999No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT257920.6447999999999999No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC238310.595775No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC230720.5768No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCT211300.52825No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA205920.5147999999999999No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC196040.4901No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG195830.489575No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA186670.466675Illumina PCR Primer Index 6 (100% over 28bp)
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT183640.45909999999999995No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT178050.44512500000000005No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCG175960.4399No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC165180.41295000000000004No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT157270.39317500000000005No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATATCT151380.37845No Hit
GAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC148270.370675No Hit
CGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCTC116340.29085No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT112660.28165No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGTA92970.232425No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT92300.23075No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA90200.2255No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC90060.22515000000000002No Hit
AAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC80390.200975No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA76590.191475Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA76130.190325Illumina PCR Primer Index 6 (96% over 29bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC74950.18737499999999999No Hit
GCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC71660.17915No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA71150.177875Illumina PCR Primer Index 6 (100% over 28bp)
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT68720.17179999999999998No Hit
AGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCGT64600.16149999999999998No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCGT63440.1586No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC61970.154925No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC61840.15460000000000002No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGT60140.15035No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT58200.1455No Hit
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG56510.14127499999999998No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA55620.13905Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA54460.13615000000000002Illumina PCR Primer Index 6 (96% over 29bp)
TATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCG52880.1322No Hit
ACAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT52230.130575No Hit
TCGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATATCT50670.126675No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCG50590.126475No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC46800.117No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA46240.1156Illumina PCR Primer Index 6 (96% over 29bp)
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG45560.1139No Hit
GGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA44970.112425No Hit
CAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT44690.11172499999999999No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATCT42560.10640000000000001No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATATCTC42340.10585No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTC40900.10225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)