FastQCFastQC Report
Thu 22 Jun 2023
ERR2382547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA64877816.21945Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1580913.952275Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA983472.458675Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA717571.793925No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG611701.52925No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA439121.0977999999999999Illumina PCR Primer Index 6 (100% over 28bp)
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG364110.910275No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG323560.8089000000000001No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA313180.7829499999999999Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCCTG311040.7776No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG310390.775975No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC304150.760375No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA235660.58915Illumina PCR Primer Index 6 (96% over 29bp)
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCC225050.562625No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCC213800.5345No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT211020.52755Illumina PCR Primer Index 6 (95% over 21bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA210120.5253Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA192890.482225No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT177890.444725TruSeq Adapter, Index 6 (95% over 22bp)
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC175690.43922500000000003No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT171140.42785No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCTGTAGGCACCAT162570.40642500000000004No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT159070.397675Illumina PCR Primer Index 6 (95% over 21bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCC154700.38675No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG151660.37915000000000004No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCTGT151350.37837499999999996No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATC147100.36775No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG146830.367075No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG146650.366625No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA143230.35807500000000003Illumina PCR Primer Index 6 (96% over 29bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC136630.341575No Hit
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT132980.33245Illumina PCR Primer Index 6 (95% over 21bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA131410.328525Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC130490.326225No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC129920.3248No Hit
CTCGCCCCCGGGAGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT114610.28652500000000003Illumina PCR Primer Index 6 (95% over 22bp)
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC112750.281875No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCT111310.278275No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT110570.27642500000000003RNA PCR Primer, Index 6 (95% over 22bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT101730.25432499999999997No Hit
GCGGCGCTACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT100440.25110000000000005Illumina PCR Primer Index 6 (96% over 27bp)
GGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCTT97630.24407500000000001No Hit
ATCCGGGTAGGACCTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT97500.24375Illumina PCR Primer Index 6 (95% over 22bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC95760.23939999999999997No Hit
GCCCCCTATGAGGAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT94470.23617500000000002Illumina PCR Primer Index 6 (95% over 21bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG91900.22975No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT90840.2271No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA89880.22469999999999998Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA89780.22444999999999998Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC87680.2192No Hit
CCCTCACTGGGGTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT87230.218075Illumina PCR Primer Index 6 (95% over 21bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA84740.21185No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCCTGTAGGCACCAT80640.2016No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCCT76610.191525No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCT75150.187875No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT74710.186775No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA73430.183575Illumina PCR Primer Index 6 (100% over 28bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCT71950.179875No Hit
TCCCGCTAGAGACCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT70390.175975Illumina PCR Primer Index 6 (95% over 22bp)
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA69290.17322500000000002No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCCT65290.163225No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG64340.16085No Hit
ATCCGATTGGAAGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT64230.160575Illumina PCR Primer Index 6 (95% over 21bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCT62460.15614999999999998No Hit
ACCTCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT62090.155225Illumina PCR Primer Index 6 (95% over 21bp)
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG58030.145075No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAAGTCCTGGCGGGGAAGCCT56970.142425No Hit
TCCTGAGTGCGAGCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT56620.14155Illumina PCR Primer Index 6 (95% over 21bp)
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC56360.1409No Hit
CTCGCCCCCGGGAGACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT55700.13925Illumina PCR Primer Index 6 (95% over 21bp)
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC55660.13915No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA54470.136175No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC52280.1307No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA52140.13035No Hit
GTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG51440.1286No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCCTCTT49440.12359999999999999Illumina PCR Primer Index 6 (95% over 21bp)
GCGGCGCTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT48930.12232499999999999Illumina PCR Primer Index 6 (96% over 28bp)
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC48700.12175000000000001No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC48640.12160000000000001No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCCTGTAGGCACCAT48040.1201No Hit
TCCACAGAATTCGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT46190.115475Illumina PCR Primer Index 6 (95% over 21bp)
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT45950.114875No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACCT44990.112475No Hit
ATCCTGTCTGTGACGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT44610.11152499999999999Illumina PCR Primer Index 6 (95% over 21bp)
TCCTGAGTGCGAGCTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT44530.11132500000000001Illumina PCR Primer Index 6 (95% over 22bp)
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCCTGTAGGCACCA44170.110425No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCT44170.110425No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG42900.10725000000000001No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC42650.106625No Hit
ATCCCTTAGCTCTCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT41920.1048Illumina PCR Primer Index 6 (95% over 21bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCTG41250.10312500000000001No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCT40990.10247500000000001No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC40850.10212500000000001No Hit
ACCTCCCCTGGAGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT40680.10169999999999998Illumina PCR Primer Index 6 (95% over 21bp)
CTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCC40300.10074999999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)