FastQCFastQC Report
Thu 22 Jun 2023
ERR2382549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526845
Total Bases78.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA21231840.29989845210641Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA489339.287930985394185Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA392417.448300733612352Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA330876.280215243572587Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA191623.6371228729512475Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA130702.4808055500194555Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA72021.367005475993888Illumina PCR Primer Index 6 (100% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA67271.2768461312150632Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA59071.1212026307547762Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC56031.0635006500963282No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA40960.7774582657138248Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA26520.503373857586197Illumina PCR Primer Index 6 (96% over 29bp)
TGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG24750.46977763858440336No Hit
GACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA23240.44111645740208216Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT20900.39670111702682953No Hit
ACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19060.3617762339967163Illumina PCR Primer Index 6 (96% over 29bp)
GGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG18570.3524755857984796No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT16240.3082500545701297No Hit
CTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15020.28509333864798947Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA12140.23042830433998612Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA11890.22568307566741644No Hit
CTGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10180.19322571154703944Illumina PCR Primer Index 6 (100% over 28bp)
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT10170.19303590240013666No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA8130.15431483643196764No Hit
TCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7910.1501390352001063Illumina PCR Primer Index 6 (100% over 28bp)
TCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA7610.1444447607930226Illumina PCR Primer Index 6 (96% over 29bp)
AGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCA7230.13723201321071662No Hit
CTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6940.13172754795053573Illumina PCR Primer Index 6 (96% over 29bp)
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT6500.123375945486813No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC6280.11920014425495164No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG6010.11407529728857634No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC5990.11369567899477076No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5960.11312625155406239Illumina PCR Primer Index 6 (95% over 21bp)
ATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCG5610.10648293141246477No Hit
TCGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5590.10610331311865918Illumina PCR Primer Index 6 (100% over 28bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG5450.10344598506202014No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC5300.1005988478584783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)