ERR2382555_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR2382555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186852
Total Bases	161.4 Mbp
Sequences flagged as poor quality	0
Sequence length	136
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	353545	29.788465621661338	Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	166723	14.04749707629932	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	135687	11.432512225618696	Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	70936	5.9768193506856795	Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA	56829	4.788212852150058	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	32999	2.780380367560572	Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	21385	1.8018253328974463	Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	10921	0.9201652775577748	Illumina PCR Primer Index 6 (96% over 29bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	10124	0.8530128440614331	Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	8552	0.7205616201514595	Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7999	0.6739677735724421	Illumina PCR Primer Index 6 (100% over 28bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	6296	0.5304789476699706	Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA	5675	0.4781556588353055	Illumina PCR Primer Index 6 (96% over 29bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	5143	0.43333119883523813	Illumina PCR Primer Index 6 (96% over 29bp)
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4875	0.4107504558276853	Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA	4333	0.3650834307900227	Illumina PCR Primer Index 6 (96% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	3829	0.322618152895222	Illumina PCR Primer Index 6 (100% over 28bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA	3061	0.25790915800790665	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2841	0.23937272718081107	Illumina PCR Primer Index 6 (100% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2505	0.21106254191761062	Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA	2487	0.20954592484993917	Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAA	2236	0.18839754240629836	Illumina PCR Primer Index 6 (96% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	1913	0.1611826916919717	Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1790	0.1508191417295501	Illumina PCR Primer Index 6 (100% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1773	0.14938678116563817	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1598	0.13464189300772125	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1589	0.13388358447388554	Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA	1566	0.13194568488741645	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1503	0.12663752515056637	Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGAAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1481	0.12478388206785682	Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	1468	0.12368854751898298	Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA	1462	0.12318300849642584	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1457	0.12276172597762822	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1390	0.11711654022574002	Illumina Single End Adapter 1 (96% over 27bp)
AGCTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1283	0.10810109432347083	Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG	1261	0.10624745124076128	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1260	0.10616319473700174	RNA PCR Primer, Index 37 (96% over 27bp)
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	1226	0.10329847360917789	TruSeq Adapter, Index 6 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content