FastQCFastQC Report
Fri 23 Jun 2023
ERR2382556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA131374732.843675Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA47000011.75Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3261978.154925Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA764171.910425Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA677381.6934500000000001Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA633341.58335Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA520841.3021Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA462921.1573Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT289230.723075No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA269250.673125Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA216600.5415Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA208510.521275Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA191430.47857500000000003Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT185040.4626No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA162750.40687500000000004No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT117610.29402500000000004No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA113980.28495Illumina PCR Primer Index 6 (100% over 28bp)
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA98920.2473No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA87130.21782500000000002Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTCTGCCGTCTTCTGCTTGAAAA85800.21450000000000002RNA PCR Primer, Index 37 (96% over 27bp)
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG79930.199825No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT72060.18015No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATATCTCGTAT71650.179125No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA70900.17725Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA70420.17604999999999998No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA69620.17405Illumina PCR Primer Index 6 (96% over 29bp)
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA68780.17195000000000002No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA62890.157225Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG62790.156975No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA60740.15185No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT58920.1473No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA57370.143425Illumina PCR Primer Index 6 (96% over 29bp)
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA56500.14125No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA55880.1397Illumina PCR Primer Index 6 (96% over 28bp)
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG54950.137375No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG53510.133775No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT52830.132075No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52190.13047499999999998Illumina PCR Primer Index 6 (96% over 29bp)
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTAT51540.12885No Hit
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48950.122375Illumina PCR Primer Index 6 (100% over 28bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48740.12185Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48090.120225Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA47550.118875Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC47510.118775No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG46740.11685000000000001No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATATC46500.11624999999999999No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA46240.1156Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG46140.11535000000000001No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATATCTCGTAT45420.11355No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAA44690.11172499999999999Illumina PCR Primer Index 6 (96% over 28bp)
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATATCTCGTA40930.10232500000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)