FastQCFastQC Report
Fri 23 Jun 2023
ERR2382557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA129817132.454274999999996Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA46898811.7247Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3270028.17505Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA772641.9316Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA684861.71215Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA638591.5964749999999999Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA525291.313225Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA481231.203075Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT300910.752275No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA266310.665775Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA226310.5657749999999999Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA211050.527625Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT196440.4911No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA189970.474925Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA178070.445175No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT123840.3096No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA111710.279275Illumina PCR Primer Index 6 (100% over 28bp)
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA107970.26992499999999997No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA85500.21375Illumina PCR Primer Index 6 (100% over 28bp)
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG83760.2094No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA77100.19275No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA73930.18482500000000002No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT73030.182575No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA72430.181075Illumina PCR Primer Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA68000.16999999999999998Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTCTGCCGTCTTCTGCTTGAAAA67660.16915RNA PCR Primer, Index 37 (96% over 27bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA65990.16497499999999998No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA63220.15805Illumina PCR Primer Index 6 (96% over 28bp)
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG62610.156525No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA61920.1548Illumina PCR Primer Index 6 (96% over 29bp)
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA61220.15305No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA60090.150225Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATATCTCGTAT57790.14447500000000002No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG57460.14365No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT57380.14345No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT54630.136575No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG54090.13522499999999998No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTAT53580.13394999999999999No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA51960.12990000000000002Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC49820.12455000000000001No Hit
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA49070.12267499999999999Illumina PCR Primer Index 6 (100% over 28bp)
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG48830.122075No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATATC48560.12140000000000001No Hit
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48180.12045Illumina PCR Primer Index 6 (100% over 28bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48110.120275Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA48040.1201Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAA47430.118575Illumina PCR Primer Index 6 (96% over 28bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG46960.11739999999999999No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA44830.112075Illumina PCR Primer Index 6 (96% over 29bp)
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATATCTCGTA44640.1116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)