FastQCFastQC Report
Fri 23 Jun 2023
ERR2382558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA128653832.16345Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA46406611.60165Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3268368.1709Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA772991.9324750000000002Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA712571.781425Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA633631.5840750000000001Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA519651.2991249999999999Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA492901.23225Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT325870.8146749999999999No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA265590.663975Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA225690.564225Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA214440.5361Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT211610.529025No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA188970.47242500000000004Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA188420.47105No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT135540.33885No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA114950.287375No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA110530.276325Illumina PCR Primer Index 6 (100% over 28bp)
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG89930.22482499999999997No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA85450.213625Illumina PCR Primer Index 6 (100% over 28bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA82930.207325No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA78500.19624999999999998No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT75580.18894999999999998No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA71530.17882499999999998Illumina PCR Primer Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA71080.1777Illumina PCR Primer Index 6 (96% over 29bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA69110.17277499999999998No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG67690.169225No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTCTGCCGTCTTCTGCTTGAAAA66900.16724999999999998RNA PCR Primer, Index 37 (96% over 27bp)
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA65420.16355No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG61760.1544No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA60800.152Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATATCTCGTAT60770.151925No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA60300.15075Illumina PCR Primer Index 6 (96% over 29bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT58200.1455No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG56680.14170000000000002No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTAT54850.137125No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT53160.13290000000000002No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG51750.129375No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG50730.126825No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50680.12669999999999998Illumina PCR Primer Index 6 (96% over 29bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATATC49600.124No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC49430.123575No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAA47560.11889999999999999Illumina PCR Primer Index 6 (96% over 28bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA47510.118775Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA47290.118225Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA46930.117325Illumina PCR Primer Index 6 (100% over 28bp)
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATATCTCGTA46690.11672500000000001No Hit
TGTAGGCACCATCAATATCTCGTCTGCCGTCTTCTGCTTGAAAAAAAAAA46420.11605000000000001Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA45680.1142Illumina PCR Primer Index 6 (100% over 28bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA45680.1142Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA40730.101825Illumina PCR Primer Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)