FastQCFastQC Report
Fri 23 Jun 2023
ERR2382561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802947
Total Bases107.6 Mbp
Sequences flagged as poor quality0
Sequence length121-135
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA39696049.43788319777022Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA629977.845723316731988Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA302423.766375613832544Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA217512.708896103976975Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA205012.5532195773818196Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA118981.4817914507433243Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA115141.4339676217732926Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA105221.3104227302673777RNA PCR Primer, Index 44 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC66730.831063569575576TruSeq Adapter, Index 6 (96% over 26bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT53030.660442096427286No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA48380.6025304285338883No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA41930.5222013408107883Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA39180.48795250495985415Illumina PCR Primer Index 6 (100% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA30860.38433420885811886Illumina PCR Primer Index 6 (100% over 28bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC28170.3508326203348415No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC28050.34933812567952804No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA26200.3262979997434451No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC24690.30749227533075035Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24450.3045032860201234RNA PCR Primer, Index 37 (96% over 27bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC23260.2896828806882646No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA22500.2802177478712792Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA22030.27436431047130133Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA20660.2573021631564723Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA19830.24696524179055407Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA19130.23824735630122534RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC19020.236877402867188Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17690.22031342043746352Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC17360.21620356013535139No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC16210.20188131968859716No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC15910.19814508305031342No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA14650.1824528891695218No Hit
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13320.16588890673979728Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC12000.14944946553134889No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG11900.14820405331858766No Hit
ACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA11480.14297332202499044RNA PCR Primer, Index 6 (96% over 29bp)
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC11090.1381162143952216No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10860.13525176630587074No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC10610.13213823577396766No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG10400.12952287012716904No Hit
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10250.12765475180802718RNA PCR Primer, Index 32 (96% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA9730.12117860830166874Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9710.12092952585911647Illumina PCR Primer Index 6 (96% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9580.11931048998252687Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9270.11544971212296702No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA8850.11021898082936982Illumina PCR Primer Index 6 (96% over 29bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8840.11009443960809369No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG8320.10361829610173523No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC8040.10013114190600375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)