FastQCFastQC Report
Fri 23 Jun 2023
ERR2382567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673484
Total Bases90.3 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA258303.835280422400532No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC224443.332521633773037No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT210803.129992694703957No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG203223.0174436215262723No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC194932.8943523528398596No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA179942.671778394141509No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC134671.9996020692399523TruSeq Adapter, Index 6 (96% over 26bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC129451.9220946600067705No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC109811.6304767448075974No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC106171.5764294326220074No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG92541.3740489751798113No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG91181.3538554739236568No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC87621.3009960147531343No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC82781.2291309073415255No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC76781.1400419312114318No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC73101.0854006925183077No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC72411.075155460263347No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA61140.9078166667656545No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA53960.801206858663309No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG52970.7865071776018435No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG49940.7415172446561462No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG48540.7207298168924577No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC47990.7125633274138659No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG47710.7084058418611281No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCACTGTAGGCACC46980.6975666830986333No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC44890.6665340230799841No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC44740.6643067986767317No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC44240.6568827173325573No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG43850.6510919338841012No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC41090.6101110048642581No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC40990.6086261885954232No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA39770.5905114301156374No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG39060.5799692346069097No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC38520.5719512267552013No Hit
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA36640.5440366809011052No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC35940.5336429670192611No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG35700.5300794079740573No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC33430.4963740786715052No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC32680.4852379566552435No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA30580.4540568150097107No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA28590.4245089712598963No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC28260.41960907757274113No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC27200.4038700251230913No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC25400.37714333228406316No Hit
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC24190.35917705543116096No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT23240.3450713008772294No Hit
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC23180.3441804111159285No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC23100.3429925581008606No Hit
TTGCATTTGTAATGCAAAGGTCGTTAGTTCATTTCTGACAGGTGGCACCA22620.3358654400104531No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAAGTCCTGGCGGGGAAGCCA21700.3222051303371721No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC20840.30943571042519197No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC17920.2660790753752131No Hit
ATCTGGAGGTCCTGTGTTCGGTCCACAGAATTCGCACCACTGTAGGCACC17200.2553883982396018No Hit
ACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTG16160.23994630904371891No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC15830.23504641535656376No Hit
ACATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGC15380.22836474214680677No Hit
TGGGAGAGGTCTCCGGTTCGTTTCCGGACTCGTCCACCACTGTAGGCACC15360.22806777889303975No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA15080.22391029334030207No Hit
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG14320.21262568969715687No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA13860.20579553486051635Illumina PCR Primer Index 6 (96% over 29bp)
ATCTGGAGGTCCTGTGTTCGTTCCACAGAGTTCGCACCACTGTAGGCACC13800.20490464509921544No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCATGTCCTGGCGGGGAAGCCA13230.19644119236685653No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG13100.19451093121737115No Hit
TCCACAGAGTTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC12450.18485962546994436No Hit
TCTGACTTCTAATCAGAAGATTATGGGTCCCCCCCATCGTGAGTGCCACT12210.1812960664247406No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG12060.17906884202148826No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCA11090.1646661242137898No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGTGCCCCCTATGAGGAGCCA10980.16303282631807142No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT10950.16258738143742094No Hit
TCAGATTTAGGCTCTGATATCTTCGGATGCAAGGGTTCGAATCCCTTAGC10850.16110256516858604No Hit
TCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGC10090.14981796152544086No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCCA9990.14833314525660596No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT9870.14655136573400407No Hit
ATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCA9860.14640288410712057No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCA9770.1450665494651692No Hit
TGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACTGTAGGC9760.14491806783828567No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGTGCCAGATCGGGG9580.14224539855438287No Hit
ATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCAC9270.13764246812099473No Hit
AAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGC8960.13303953768760654No Hit
TAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCAC8950.13289105606072305No Hit
TCAGTCTCATAATCTGAAGGTCGAGAGTTCGAACCTCCCCTGGAGCACCA8490.12606090122408253No Hit
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8340.12383367682083019No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACCA8320.12353671356706321No Hit
ATCACATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGG7860.11670655873042271No Hit
TCCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7810.11596415059600526No Hit
AAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCC7690.11418237107340339No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA7690.11418237107340339Illumina PCR Primer Index 6 (96% over 29bp)
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGAGCCCCCTATGAGGAGCCA7610.11299451805833545No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG7550.11210362829703452No Hit
TGCGGGAGGTCCCGGGTTCGAGTCCCGGCTCGCCCCCCACTGTAGGCACC7330.10883703250559776No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGGGCCCCCTACAGGGCTCCA7150.10616436322169495No Hit
ATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGC6960.10334321231090865No Hit
ATGCCTTGCTAAGGCATTGGGTTTTACCTGCGCAGGTTCGAATCCTGTCT6910.1026008041764912No Hit
ACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACC6820.10126446953453981No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGGCCCCCATCGTGAGTGCCA6740.10007661651947188No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAGCCCTCACTGGGGTCGCCA6740.10007661651947188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)