FastQCFastQC Report
Thu 22 Jun 2023
ERR2382568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491247
Total Bases468 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA112797532.30865647718423Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA2680097.6765980751290295Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2051435.875923416475546Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1152253.3003966777486666Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1024772.935254938994577Illumina PCR Primer Index 6 (100% over 28bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA817962.3428877991159034No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA725702.0786269204098136Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG481631.3795357360851295No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT473861.3572800778632965No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA336610.9641540687324615Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA319930.9163774433604955Illumina PCR Primer Index 6 (96% over 29bp)
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG317310.9088729614375608No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA280680.8039534298203479Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA270110.773677714581638Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA269850.7729329950014995Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA257400.7372723843371723Illumina PCR Primer Index 6 (100% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA251420.7201438339939855Illumina PCR Primer Index 6 (96% over 29bp)
TCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA239160.685027441484375Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC210360.6025354264536426No Hit
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG206620.591822921723957No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC187500.5370573895229985No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA173920.49816011299114615Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT117310.33601174594636246No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG113520.3251560259128042Illumina PCR Primer Index 6 (96% over 29bp)
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC99700.2855713159223624No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA87390.25031170810887915Illumina PCR Primer Index 6 (96% over 29bp)
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT75350.215825462936309No Hit
GCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA73320.21001092159907334Illumina PCR Primer Index 6 (96% over 29bp)
CAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA64830.18569296300147198No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG63690.18242765407317216No Hit
TTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAA56500.16183329337626354No Hit
CAAGCAGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA56000.16040114033753555Illumina PCR Primer Index 6 (96% over 29bp)
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG55490.158940344238033No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC54220.1553026755196639No Hit
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA53010.15183686516594214TruSeq Adapter, Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT50580.14487660139772407No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA49610.14209822450259177Illumina PCR Primer Index 6 (96% over 29bp)
ACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTC49160.14080928676773657No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48130.13785905150795688Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT47040.13473695788352988No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAA44850.12846412757390124No Hit
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA44750.12817769696615564Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAA44400.12717518983904605TruSeq Adapter, Index 6 (96% over 28bp)
TTGGATTTGAGTAAGAGCAAGCTGTAGGCACCATCAATATCTCGTATGCC43830.12554253537489612No Hit
ATTTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGG43790.12542796313179788No Hit
TTGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA42960.12305058908750942No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC41850.11987120934153328Illumina PCR Primer Index 6 (96% over 29bp)
TTAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGT40290.11540289186070193No Hit
TCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGG39860.11417124024739585No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT39460.11302551781641344No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA38370.10990342419198641Illumina PCR Primer Index 6 (96% over 29bp)
TTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGA37680.10792705299854177No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT37300.1068386166891085No Hit
TCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAAC36780.1053491775288314No Hit
ACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACCA36650.10497681773876211No Hit
AAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAC36140.10351602163925955No Hit
TAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAG35440.10151100738504035No Hit
ACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAG35060.10042257107560708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)