FastQCFastQC Report
Thu 22 Jun 2023
ERR2382569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149510
Total Bases154.2 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15367613.368826717470922Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA946338.232464267383493Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA789836.871014606223522Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA551314.796043531591722Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA506504.40622526119825Illumina PCR Primer Index 6 (100% over 28bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC329932.8701794677732253TruSeq Adapter, Index 6 (96% over 26bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG150171.306382719593566No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC143191.2456611947699454No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC140141.2191281502553262No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC126231.0981200685509478No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA122441.0651494984819618No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG120401.0474028064131673No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA112020.9745021791893937No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT104390.9081260711085593No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC101510.8830719175996729No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG97180.8454036937477708No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC91280.7940774764899827No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA86840.7554523231637829Illumina PCR Primer Index 6 (100% over 28bp)
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC79670.6930779201572844No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC73570.6400118311280458No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG61130.5317918069438282No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC58650.5102173969778427No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC54630.4752459743716888No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG54270.472114205183078No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC53680.46698158345729923No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC53530.4656766796287114No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT51340.446625083731329No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC46680.4060860714565337No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG44550.38755643709058646No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC41260.35893554645022663No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA40180.3495402388843942Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC37150.32318118154692No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA36420.3168306495811259No Hit
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34650.30143278440378946RNA PCR Primer, Index 22 (96% over 27bp)
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA32850.28577393846073545No Hit
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC31620.2750737270663152No Hit
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG29050.252716374803177No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC28720.24984558638028376No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC28690.24958460561456622No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA28310.24627884924881038Illumina PCR Primer Index 6 (96% over 29bp)
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG27770.24158119546589416No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA27520.2394063557515811Illumina PCR Primer Index 6 (96% over 29bp)
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG27400.23836243268871085No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC27080.23557863785439015No Hit
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC25450.22139868291706902No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA25030.21774495219702306Illumina PCR Primer Index 6 (96% over 29bp)
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCCA23640.20565284338544249No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC21140.18390444624231195No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC20950.18225156805943402No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA20170.17546606815077728Illumina PCR Primer Index 6 (96% over 29bp)
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC19620.17068142077928855No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAGCCCTCACTGGGGTCGCCA18500.16093813885916608No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC18490.16085114527059358No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC18120.15763238249341022No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA17980.15641447225339494Illumina PCR Primer Index 6 (96% over 29bp)
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTTTTAACCGAAATGTCAG17780.15467460048194448No Hit
GGGGCGTGTGGTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCT17510.15232577359048638No Hit
ATCTGGAGGTCCTGTGTTCGGTCCACAGAATTCGCACCACTGTAGGCACC17490.15215178641334132No Hit
GTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG17430.1516298248819062No Hit
CAAGCTGTAGGCACCCTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA17040.14823707492757784Illumina PCR Primer Index 6 (96% over 29bp)
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA16840.14649720315612738No Hit
GCAGACTTAAGATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCA15430.13423110716740175No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCATGTCCTGGCGGGGAAGCCA14810.12883750467590538No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA14550.1265756713730198Illumina PCR Primer Index 6 (96% over 29bp)
CTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCA14360.12492279319014188No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT14250.12396586371584414No Hit
TGGCCATCTTAGTATAGTGGTTAGTACACATCGTTGTGGCCGATGAAACC13780.11987716505293561No Hit
TTGCATTTGTAATGCAAAGGTCGTTAGTTCATTTCTGACAGGTGGCACCA13550.11787631251576759No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA13220.11500552409287436No Hit
GTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG13080.11378761385285904No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA12940.11256970361284374TruSeq Adapter, Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA12690.11039486389853068Illumina PCR Primer Index 6 (100% over 28bp)
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA12680.11030787030995816No Hit
CCACATTGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACT12630.10987290236709554No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA11870.10326138963558387RNA PCR Primer, Index 37 (96% over 27bp)
GCTGTAGGCACCATCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11860.10317439604701133Illumina PCR Primer Index 6 (96% over 28bp)
CTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG11820.10282642169272124No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT11560.10056458838983567RNA PCR Primer, Index 6 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)