FastQCFastQC Report
Thu 22 Jun 2023
ERR2382570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140778
Total Bases555.4 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA88612521.399963968123863Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA80478319.43555051731824Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA63358815.301182531398688Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA3848199.293398486951004Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3093257.470214534563311Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA478441.1554350414342425Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA454841.098440921005666Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC382370.923425501198084No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA208980.5046877664052504Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA192770.46554053368714765Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA191050.4613867249101497RNA PCR Primer, Index 22 (96% over 27bp)
CAAGCTGTAGGCACCCTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA172160.4157672785162595Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA160520.3876566191184362No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA132480.31993987603295804Illumina PCR Primer Index 6 (96% over 29bp)
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC112270.2711326229032322No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA98570.23804705299342296Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT91820.22174576854880895No Hit
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA91790.22167331839572177Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA89720.21667425783270677Illumina PCR Primer Index 6 (96% over 29bp)
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA86040.20778703905401352Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA80580.19460111119214796Illumina PCR Primer Index 6 (100% over 28bp)
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA71830.17346981654172236Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA67370.16269889378276256Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAA65570.15835188459753216TruSeq Adapter, Index 6 (96% over 28bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA63680.1537875249530402RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA59990.1448761561233179RNA PCR Primer, Index 40 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA59850.14453805540891107TruSeq Adapter, Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA56570.1366168386713801Illumina PCR Primer Index 6 (100% over 28bp)
TCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA54450.13149702785321984Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA53910.13019292509765074Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC53200.12847827147458762No Hit
GCTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAA51970.12550781519801352Illumina PCR Primer Index 6 (96% over 28bp)
TTGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA50170.12116080601278309No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA48660.11751414830739537Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCGTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA48410.11691039703166893TruSeq Adapter, Index 6 (96% over 31bp)
GTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC48030.11599269509256474No Hit
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA47940.1157753446333032RNA PCR Primer, Index 37 (96% over 27bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA45650.11024498294764896Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA45530.10995518233530027Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC45180.10910993054928325Illumina PCR Primer Index 6 (96% over 29bp)
CTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC43460.1049561217722853No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG43240.10442482064964603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)