FastQCFastQC Report
Thu 22 Jun 2023
ERR2382572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524080
Total Bases125.6 Mbp
Sequences flagged as poor quality0
Sequence length35-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1502365.952109283382461Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT922243.653766917054927No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA453561.796931951443694Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA436921.7310069411429114Illumina PCR Primer Index 6 (100% over 28bp)
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC411221.6291876644163417No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT396411.5705128205128205No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT363741.4410795220436752No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC200350.7937545561154955No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT198900.788009888751545No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT154100.6105194764032835No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG150630.596771893125416No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCGT143220.5674146619758487No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA137330.5440794269595258Illumina PCR Primer Index 6 (96% over 29bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGT134730.5337786441000285No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA132610.5253795442299768Illumina PCR Primer Index 6 (96% over 29bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT126630.5016877436531331No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT120300.47660929922981843No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGT117060.4637729390510602No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA115500.4575924693353618Illumina PCR Primer Index 6 (100% over 28bp)
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCGT110720.4386548762321321No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG107440.4256600424709201No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGT98420.3899242496275871No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC91040.36068587366486005No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT86340.34206522772653797No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT85430.3384599537257139No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTAT82680.32756489493201485No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG81340.32225602991981234No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT77330.3063690532788184No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT76710.30391271275078446No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG72970.2890954327913537No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT72720.2881049729010174No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATGC66860.26488859307153495No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGT63250.2505863522550791No Hit
GCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG61120.242147633989414No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC60020.23778961047193434No Hit
TATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCG59260.23477861240531203No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG58970.23362967893252196No Hit
ACACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT49340.1954771639567684No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGT47610.18862318151564134No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGT45710.18109568634908563No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA45390.17982789768945517Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG44230.17523216379829482No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT42230.16730848467560458No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGTATG41940.1661595512028145No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCG41890.16596145922474725No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC41280.1635447370923267No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT40290.15962251592659504No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTC38830.15383823016703116No Hit
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT38700.15332319102405628No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGT35980.14254698741719754No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT35480.140566067636525No Hit
CCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT35050.13886247662514659No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCT34880.13818896389971794No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC34150.13529682101993598No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC34140.13525720262432253No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT33860.13414788754714588No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGTATGC33600.13311780926119615No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA33470.1326027701182213Illumina PCR Primer Index 6 (96% over 29bp)
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC33340.13208773097524643No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATATCTCGTATG33230.13165192862349848No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTC32440.12852207537003582No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT31940.12654115558936324No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGTAT31430.12452061741307723No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTC31250.12380748629203511No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC30230.1197664099394631No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCTTAT29640.11742892459826948No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA28800.11410097936673956Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT27330.10827707521156224No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC26310.10423599885899021No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCG26180.10372095971601535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)