FastQCFastQC Report
Thu 22 Jun 2023
ERR2382574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846041
Total Bases247.5 Mbp
Sequences flagged as poor quality0
Sequence length46-135
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA18673910.115647485619226Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC1193686.466161910813466No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT627313.398136877783321No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT371952.0148523245150027No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA362971.9662076844447116Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA268411.4539763743058793Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA262631.4226661271336876Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT226461.22673331740736No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA146860.7955402940671417Illumina PCR Primer Index 6 (100% over 28bp)
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG146780.795106934244689No Hit
TTGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTA135170.7322155900112728No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC132510.7178063759147278No Hit
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG130710.7080557799095469No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC125710.6809707910062669No Hit
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG124150.6725202744684435No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA111070.6016659434974629Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG110380.5979282150288103No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT107500.582327261420521No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA101250.5484710252914209No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT99530.5391537891086926No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG93850.5083852417145664No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC78700.4263177253376279TruSeq Adapter, Index 6 (96% over 26bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG78460.42501764587027047No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA77760.4212257474238113Illumina PCR Primer Index 6 (96% over 29bp)
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCT74680.4045413942593908No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG70180.38016490424643873No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA69050.37404369675429744Illumina PCR Primer Index 6 (96% over 29bp)
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAA65710.3559509241669064No Hit
TAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTT63850.3458753082948862No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA63590.3444668888719156Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA63030.3414333701147483No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC61890.33525799264480044No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG57210.30990644303133025No Hit
TCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAG57120.30941891323107124No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC56030.3035143856501562No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC54410.29473884924549343No Hit
ATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTA51290.2778378161698467No Hit
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT51210.27740445634739425No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTCT51190.27729611639178114Illumina PCR Primer Index 6 (95% over 21bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG50970.2761043768800368No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC50590.2740459177233875No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC50300.27247498836699724No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT48040.2602325733827147No Hit
ACCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTC48010.260070063449295No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG47290.25616982504722263No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG45250.24511914957468442No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC44510.24111057121699897No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC43350.23482685379143797No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA42040.2277305866987786Illumina PCR Primer Index 6 (96% over 29bp)
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGT41190.223126138585221No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA39110.2118587832014565No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT38120.2064959553986071No Hit
ACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTC38000.20584591566492838No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG36990.20037474790646578No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAA36210.19614948963755408No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC34280.185694683920888No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC33930.1837987346976584No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG31920.1729105691585398No Hit
TCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGTA30950.1676560813113035No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG29610.16039730428522445No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAAT29040.15730961555025053No Hit
TTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGA28440.1540594168818569No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA28120.15232597759204697No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG27610.14956330872391244No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG26520.14365878114299735No Hit
AATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG26030.14100445223047592No Hit
GGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC25880.14019190256337752No Hit
CCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAA25470.13797093347330858No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG25280.13694170389498392No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC25270.13688753391717737No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA25030.1355874544498199No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA24800.13434154496026904Illumina PCR Primer Index 6 (96% over 29bp)
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTC24440.13239142575923285No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGA24330.1317955560033607No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA24250.13136219618090822Illumina PCR Primer Index 6 (96% over 29bp)
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGACTG23440.12697442797857686No Hit
TTGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCTATACTCT23440.12697442797857686No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTGTA22750.1232366995099242No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA22660.12274916970966517Illumina PCR Primer Index 6 (96% over 29bp)
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG22530.12204495999817988No Hit
TTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTG22480.1217741101091471No Hit
TTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG22360.12112407037546835No Hit
TCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTT22060.11949897104127156No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA21830.11825306155172069No Hit
CGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTG21780.11798221166268788No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC21380.11581541255042548No Hit
TTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT21160.11462367303868115No Hit
TGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGC21090.11424448319403524No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTG20980.11364861343816307No Hit
TCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGACTGT20040.10855663552434643No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC19570.10601064656743811No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA19280.10443971721104785Illumina PCR Primer Index 6 (96% over 29bp)
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT18650.10102700860923457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)