FastQCFastQC Report
Thu 22 Jun 2023
ERR2382580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4130227
Total Bases553.9 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA104513325.304492949176886Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA102520224.821928673654014Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA50555012.240247327810312Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA3929009.512794333095979Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2160485.230898931220971Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA781571.8923172987828516Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA410570.994061585477021Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA408160.9882265551021772Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA274360.6642734164490233Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA230120.5571606596925545Illumina PCR Primer Index 6 (96% over 29bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA191840.4644781025352844Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA178780.4328575644873756TruSeq Adapter, Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA170210.41210809962745387Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA163750.39646731281355724Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA139570.3379233151107675Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA124910.30242889797582556Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA123960.3001287822678996Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA118660.287296557792102Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA103460.25049470646528627TruSeq Adapter, Index 6 (96% over 29bp)
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA97310.23560448372450232Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA88510.21429814874581954Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA84340.2042018513752392Illumina PCR Primer Index 6 (100% over 28bp)
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA82080.19872999716480472Illumina PCR Primer Index 6 (96% over 29bp)
CAACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA73250.1773510269532401Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA71030.1719760197199815RNA PCR Primer, Index 37 (96% over 27bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA64260.15558466883297215Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA58100.1406702343478942Illumina PCR Primer Index 6 (96% over 30bp)
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA56820.1375711310782676Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAA50400.12202719124154678TruSeq Adapter, Index 6 (96% over 28bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50360.12193034426437094Illumina PCR Primer Index 6 (100% over 28bp)
TCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA50150.12142189763419783Illumina PCR Primer Index 6 (96% over 29bp)
ATTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA47820.11578056121370568Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47620.11529632632782655Illumina PCR Primer Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA45140.10929181374292503Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41730.10103560893868545Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)