FastQCFastQC Report
Thu 22 Jun 2023
ERR2382581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567580
Total Bases76.1 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10433618.382606857183127Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA402527.091863702033193Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA319005.620353077980197Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA238654.204693611473273Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA237524.184784523767575Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA229664.046301842912013Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC96411.6986151731914443No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA89811.5823320060608197Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT77111.3585750026427992No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA62681.104337714507206No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC62641.1036329680397476No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC60471.0654004721801331No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA56660.9982733711547271Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC49680.8752951125832482No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA49570.8733570597977377No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA48400.8527432256245816Illumina PCR Primer Index 6 (96% over 29bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC47630.8391768561260086No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC44740.7882589238521441No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC43270.7623594911730506No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41260.7269459811832693Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA40190.708094013178759Illumina PCR Primer Index 6 (96% over 29bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC30590.5389548609887593No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC30030.5290884104443426No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC28620.5042460974664364No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC28170.4963176997075302No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC27310.4811656506571761No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC26130.4603756298671553No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA25680.45244723210824905No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA25150.4431093414144262Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC23970.4223193206244054No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC23800.41932414813770746No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC23760.4186194016702492No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA22990.40505303217167626Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC21820.38443919799852No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC21500.3788012262588534No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC20440.36012544487120757No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC18720.3298213467704993No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA18650.3285880404524472Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA18390.3240071884139681Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC16790.2958173297156348TruSeq Adapter, Index 6 (96% over 26bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA16550.2915888509108848No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG15050.26516085838119735No Hit
TTGCATTTGTAATGCAAAGGTCGTTAGTTCATTTCTGACAGGTGGCACCA14810.2609323795764474No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC13270.2337996405793016No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA12580.2216427640156454No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG12580.2216427640156454Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA12110.21336199302300998TruSeq Adapter, Index 6 (96% over 29bp)
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC11860.20895732760139538No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11850.2087811409845308Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA11700.2061383417315621Illumina PCR Primer Index 6 (96% over 29bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA11620.2047288487966454Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10930.1925719722329892No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC10310.18164840198738502No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA10270.1809436555199267TruSeq Adapter, Index 6 (96% over 29bp)
AGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA10250.18059128228619753Illumina PCR Primer Index 6 (96% over 29bp)
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA9270.1633249938334684No Hit
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA9270.1633249938334684Illumina PCR Primer Index 6 (96% over 29bp)
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC9240.16279643398287466No Hit
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC9160.161386941047958No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA8980.1582155819443955No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA8940.15751083547693717RNA PCR Primer, Index 37 (96% over 27bp)
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8750.1541632897565101No Hit
TGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACTGTAGGC7980.1405969202579372No Hit
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC7960.14024454702420805No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7950.14006836040734344No Hit
CCACATTGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACT7940.13989217379047889No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG7860.13848268085556223No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG7770.13689700130378096No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGTGCCCCCTATGAGGAGCCA7720.13601606821945805No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA7720.13601606821945805Illumina PCR Primer Index 6 (96% over 29bp)
TCCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7500.13213996264843722No Hit
TCTGACTTCTAATCAGAAGATTATGGGTCCCCCCCATCGTGAGTGCCACT6220.1095880756897706No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT6020.10606434335247894No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCCA5860.10324535748264561No Hit
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA5850.10306917086578105Illumina PCR Primer Index 6 (96% over 30bp)
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA5830.10271679763205187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)