FastQCFastQC Report
Thu 22 Jun 2023
ERR2382583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416091
Total Bases55.7 Mbp
Sequences flagged as poor quality0
Sequence length121-135
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15443337.11519835805148Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA161453.880160830203008Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA126273.0346727038075807Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA113032.7164730792062315Illumina PCR Primer Index 6 (100% over 28bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC103352.483831661823976No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA93782.2538338969119738Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA89382.1480877981018573Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA87352.099300393423554Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC74531.7911947146177158No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC66141.5895561307502446No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA49561.1910856038703073Illumina PCR Primer Index 6 (96% over 29bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC36960.8882672300049749No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA33760.8113609763248905No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC29000.6969629239757649No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC28730.6904739588215079TruSeq Adapter, Index 6 (96% over 26bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC26430.6351975889889472No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA22270.5352194592048374Illumina PCR Primer Index 6 (100% over 28bp)
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC21830.5246448493238258No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC20980.5042166256900533No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC20960.5037359616045529No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA19370.46552316680726097Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA19300.4638408425080091Illumina PCR Primer Index 6 (96% over 29bp)
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC19270.4631198463797582No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT19120.45951486573850436No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC19110.45927453369575405No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC17310.4160147660007066No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC17210.41361144557320395No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC16960.40760314450444735No Hit
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC14810.35593175531314064No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC14580.35040411832988455No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA14540.3494427901588835No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG12620.30329903795083285No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC12500.3004150534378297No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC12000.2883984513003165No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC10820.2600392702557854No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG10490.25210831284502666No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9770.2348044057670077No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC8760.21053086944923105Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA8480.20380157225222367No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC8450.2030805761239729No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8450.2030805761239729No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA8150.19587061484146495TruSeq Adapter, Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA7990.19202530215746075Illumina PCR Primer Index 6 (96% over 29bp)
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7600.18265235249020048No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7550.18145069227644914Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7180.1725584066946894Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6690.16078213659992646RNA PCR Primer, Index 37 (96% over 27bp)
TCCTGGGCGAAATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT6600.1586191482151741Illumina PCR Primer Index 6 (95% over 24bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6280.15092852284716565Illumina PCR Primer Index 6 (100% over 28bp)
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC6200.14900586650516354No Hit
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA5690.1367489323249001Illumina PCR Primer Index 6 (96% over 30bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA5660.1360279361966493Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA5490.1319422914698948Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5220.1254533263156377Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA5170.12425166610188637RNA PCR Primer, Index 37 (96% over 27bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC4760.11439805234912555No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC4590.11031240762237107No Hit
TCAGTCTCATAATCTGAAGGTCGAGAGTTCGAACCTCCCCTGTAGGCACC4560.10959141149412027No Hit
ACGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA4480.10766875515211816Illumina PCR Primer Index 6 (96% over 29bp)
ACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA4310.10358311042536368RNA PCR Primer, Index 6 (96% over 29bp)
TCCACAGAGTTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4280.10286211429711288No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4270.10262178225436264Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)