FastQCFastQC Report
Thu 22 Jun 2023
ERR2382584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3886772
Total Bases521.1 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA195325550.25391250117064Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3184518.193199909848069Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2280475.8672595150937585Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1313083.378330398592971Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1110202.856354836352634Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC857342.2057892770659047No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA642141.6521164606516667Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA427671.1003218094603953Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA411931.0598254798583502Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA364240.9371272613881133Illumina PCR Primer Index 6 (100% over 28bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT189240.48688217369066156No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC171590.44147174056003285No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC146360.37655926305942305TruSeq Adapter, Index 6 (96% over 26bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT140790.3622286051252813No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC119940.30858511896247065No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA115460.2970588447174159Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA114360.29422873273760336Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA105990.2726941534003024RNA PCR Primer, Index 37 (96% over 27bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA104700.269375203896704Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC101060.260010106072597Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA81410.20945401479685455Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA75910.1953034548977918TruSeq Adapter, Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA69530.17888880541487898RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA69210.17806550011166078Illumina PCR Primer Index 6 (96% over 29bp)
TGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCC64160.1650727132952486No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA63690.1638634836311469Illumina PCR Primer Index 6 (100% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA55170.14194297993296237Illumina PCR Primer Index 6 (96% over 29bp)
ATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA54640.14057938052450722Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA52860.135999744775356No Hit
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA52390.13479051511125428Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA52140.13414730784311507Illumina PCR Primer Index 6 (96% over 30bp)
CTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC51640.13286089330683662No Hit
GTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC51020.13126573928185137No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTGT50260.12931038918670815No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC49750.12799824635970414No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA48800.12555405874077513Illumina PCR Primer Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC48070.12367589351780861No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC47080.1211287927359773No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC46840.12051131375856365No Hit
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA44680.11495400296184083RNA PCR Primer, Index 32 (96% over 28bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA44420.11428506740297603Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC43650.11230398901710727No Hit
ACGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA41800.10754425523287704Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41080.10569181830063612Illumina PCR Primer Index 6 (96% over 29bp)
CCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA41040.10558890513773384Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)