FastQCFastQC Report
Thu 22 Jun 2023
ERR2382585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316017
Total Bases310.5 Mbp
Sequences flagged as poor quality0
Sequence length121-135
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA834423.602823295338506Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA455221.9655296139881528No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC407711.760392950483524TruSeq Adapter, Index 6 (96% over 26bp)
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC322821.3938585079470487No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC310751.3417431737331806No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC298171.2874257831440787No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG291301.2577627884423992No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA281611.2159237173129558No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC266181.1493007175681353No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC211100.9114786290428784No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC204100.881254325853394No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC201770.8711939506488942No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC200120.8640696506113729No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT196270.8474462838571565No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC175710.7586731876320424No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA171120.7388546802549377No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC152220.6572490616433299No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC144190.6225774681273929No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG143170.6181733553769251No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC136020.5873013885476661No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA134790.5819905467015138Illumina PCR Primer Index 6 (96% over 29bp)
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC130830.5648922266114627No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG125030.5398492325401756No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT123900.5349701664538732RNA PCR Primer, Index 6 (95% over 22bp)
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC121840.5260755858009678No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA121070.5227509124501245No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC117370.5067752093356828No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC114320.4936060486602646No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA113010.4879497862062325Illumina PCR Primer Index 6 (100% over 28bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA108280.46752679276533804No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA104890.4528895945064306No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA104130.44960809873157237Illumina PCR Primer Index 6 (100% over 28bp)
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC103470.4467583787165638No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC103450.4466720235645939No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC97790.4222335155570965No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCCTGTAGGCACCATCAATATC96960.4186497767503434No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC90170.38933220265654356No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC89930.3882959408329041No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA89670.38717332385729464Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACCTGTAGGCA79620.3437798599923921No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG74890.3233568665514977No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC73400.31692340772973604No Hit
GGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCTT71260.30768340646895076No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCACTGTAGGCACC69200.29878882581604543No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC68770.2969321900486913No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC68440.2955073300411871No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG66820.2885125627316207No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC64910.28026564571849No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA62800.2711551771856597No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA61800.2668374195871619Illumina PCR Primer Index 6 (96% over 29bp)
GCCCCCTACAGGGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC61010.2634263910843487No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG60600.26165611046896464No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCC56180.24257162188360448No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT51800.22365984360218424No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG51680.22314171269036454No Hit
CCCTCACTGGGGTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT50350.2173990950843625Illumina PCR Primer Index 6 (95% over 21bp)
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC50070.21619012295678314No Hit
TTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATA47930.2069501216959979No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATC47760.2062161029042533No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG46450.2005598404502212No Hit
TCAGTCTCATAATCTGAAGGTCGAGAGTTCGAACCTCCCCTGTAGGCACC46260.19973946650650665No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC45580.19680339133952818No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATATCTCGTATGCCGT44030.19011086706185662No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43160.1863544179511636Illumina PCR Primer Index 6 (96% over 29bp)
TCAGATTTAGGCTCTGATATCTTCGGATGCCTGTAGGCACCATCAATATC42450.18328881005623016No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCA41990.18130264156092118No Hit
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT40530.17499871546711446Illumina PCR Primer Index 6 (95% over 21bp)
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG39370.16999011665285704No Hit
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA39270.16955834089300725No Hit
TGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACTGTAGGC39220.16934245301308237No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT38440.1659746020862541No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC37980.16398843359094514No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT37960.1639020784389752Illumina PCR Primer Index 6 (95% over 21bp)
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC36490.15755497476918348No Hit
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC34810.15030114200370723No Hit
AAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGC33750.1457243189492996No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACCA33290.14373815045399063No Hit
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC32970.14235646802247134No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG32420.13998170134329757No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG32400.13989534619132762No Hit
GTCCTGGCGGGGAAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT31600.13644114011252942Illumina PCR Primer Index 6 (95% over 21bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT31300.13514581283298005No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA31250.13492992495305517Illumina PCR Primer Index 6 (96% over 29bp)
AGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGG31030.13398001828138567No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC30020.12961908310690293No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG29530.12750338188363902No Hit
AGTAGAGTAGCCTTGTTGTTACGATCTGCTGAGATTAAGCCTTTGTTGTC28530.12318562428514125No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT28530.12318562428514125TruSeq Adapter, Index 6 (95% over 22bp)
CTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCACCACTG28470.1229265588292314No Hit
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC27400.11830655819883878No Hit
TGCGGGAGGTCCCGGGTTCGAGTCCCGGCTCGCCCCCCACTGTAGGCACC27390.11826338062285381No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT26570.11472281939208563No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGAGCCCCCTATGAGGAGCCA26540.1145932866641307No Hit
ACATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGC26370.1138592678723861No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGTGCCAGATCGGGG26290.11351384726450625No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT25870.1117003890731372No Hit
TCCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC24550.10600094904312016No Hit
TTTAGGGGTTCGAGCCCCCTACAGGGCTCCACTGTAGGCACCATCAATAT24110.10410113569978115No Hit
ACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTG24050.10384207024387127No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA23700.10233085508439706Illumina PCR Primer Index 6 (96% over 29bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAAGTCCTGGCGGGGAAGCCA23440.10120823810878764No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)