FastQCFastQC Report
Thu 22 Jun 2023
ERR2382588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052948
Total Bases275.1 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA59180928.827276677246573Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA28678113.969228640959244Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA24203811.789777432258392Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1810758.8202428897371Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1117995.44577846102288Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA581742.8336811258736216Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA400581.9512427981614733Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA233451.137145217511598Illumina PCR Primer Index 6 (100% over 28bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA171230.8340688609745595Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA150800.7345534324298522Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA148300.7223758224757763Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA137350.6690378908769243Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA120170.5853533552725154Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC95340.4644053332086346No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA88370.4304541566566713Illumina PCR Primer Index 6 (100% over 28bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA72850.3548555540617687No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA66500.3239244247784162Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG61110.2976694977174288No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA53060.25845759366530474Illumina PCR Primer Index 6 (100% over 28bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA52910.25772693706806016Illumina PCR Primer Index 6 (96% over 29bp)
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG51320.24998197713726797No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTGT47440.23108232648854232No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC42860.20877294505267546No Hit
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA36380.177208580051711TruSeq Adapter, Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT35250.17170430035246875No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG32690.15923442775949512No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA32360.15762698324555713Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAA31760.15470435685657893TruSeq Adapter, Index 6 (96% over 28bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC31170.15183044090741704No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG28920.14087059194874882No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC28460.13862991171719888No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA27680.13483049741152722Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA27120.13210271278181426Illumina PCR Primer Index 6 (100% over 28bp)
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG23510.11451824400812881No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC22390.10906267474870283No Hit
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA22050.10740651979494853Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA21290.10370452636890949Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA21200.10326613241056276TruSeq Adapter, Index 6 (96% over 29bp)
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG21120.10287644889203235No Hit
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA20940.10199966097533888Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA20650.10058705822066608Illumina PCR Primer Index 6 (96% over 29bp)
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA20620.10044092690121718Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)