FastQCFastQC Report
Thu 22 Jun 2023
ERR2382589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405166
Total Bases54.3 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10704526.42003524481324Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA256686.335181135633296Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA187444.62625195598841Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA176684.360681794622451Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA115302.8457471752318804Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC90142.2247671324839695TruSeq Adapter, Index 6 (96% over 26bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA35940.887043828949122No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC35100.8663115858685082No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA30090.7426585646377041No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC25730.635048350552613No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA24780.6116011708781092Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC24230.5980264879086596No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT24160.5962988009852752No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA23050.5689026226287497Illumina PCR Primer Index 6 (100% over 28bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC21550.5318807599847963No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC21020.5187997018505995No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19960.49263758558220583Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC19010.46919040590770206No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC19000.4689435934900757No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC18980.46844996865482297No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC18110.44697728832133005No Hit
ACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTG16120.39786161721368524No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16000.39489986820216894Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG15500.38255924732085117No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG15040.37120587611003886No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA14870.3670100650103908No Hit
TAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCAC14600.3603461297344792No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC14090.3477586964355351No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC14090.3477586964355351Illumina PCR Primer Index 6 (96% over 29bp)
ACATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGC13440.33171588928982193No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA13430.33146907687219557Illumina PCR Primer Index 6 (96% over 29bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC13240.32677964093729484No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG13090.3230774546728995No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG12890.31814120632037235No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC12190.3008643370865275No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC12100.29864302532789033No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12070.29790258807501124RNA PCR Primer, Index 37 (96% over 27bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA11320.27939165675303457No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC11150.2751958456533865No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG9700.23940804509756494No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC9440.23299092223927972No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG9400.23200367256877427No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9330.23027598564538979No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA8890.21941623926983014No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT8840.21818217718169836RNA PCR Primer, Index 6 (95% over 22bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8410.20756924322376508Illumina PCR Primer Index 6 (96% over 29bp)
AAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGC8310.20510111904750153No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG8030.19819037135396356No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8000.19744993410108447No Hit
TAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGG7790.192266873330931No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7610.18782424981365664Illumina PCR Primer Index 6 (96% over 29bp)
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG7580.18708381256077755No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA7090.17499000409708612TruSeq Adapter, Index 6 (96% over 29bp)
ATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCA6350.1567258851927358No Hit
TTGCATTTGTAATGCAAAGGTCGTTAGTTCATTTCTGACAGGTGGCACCA6340.15647907277510945No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA6240.1540109485988459Illumina PCR Primer Index 6 (96% over 29bp)
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC6220.1535173237635932No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA6080.15006194991682423No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5990.14784063815818702No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA5990.14784063815818702Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5930.1463597636524289Illumina PCR Primer Index 6 (95% over 21bp)
AAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCC5810.14339801464091262No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5680.14018945321176998Illumina PCR Primer Index 6 (95% over 21bp)
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5600.13821495387075913No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA5540.136734079365001Illumina PCR Primer Index 6 (100% over 28bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC5540.136734079365001No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC5530.13648726694737467No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA5320.1313042061772212No Hit
TTAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCA5310.13105739375959483No Hit
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5310.13105739375959483No Hit
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA5290.13056376892434213RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA5200.12834245716570492Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA5000.1234062088131778Illumina PCR Primer Index 6 (96% over 29bp)
AAAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTG4960.12241895914267238No Hit
TCCTGAGTGCGAGCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT4880.12044445980166155Illumina PCR Primer Index 6 (95% over 21bp)
ATCTGGAGGTCCTGTGTTCGGTCCACAGAATTCGCACCACTGTAGGCACC4770.11772952320777162No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC4690.11575502386676079No Hit
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA4660.11501458661388171Illumina PCR Primer Index 6 (96% over 30bp)
GCGGCGCTACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT4660.11501458661388171Illumina PCR Primer Index 6 (96% over 27bp)
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4610.11378052452574994No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA4610.11378052452574994Illumina PCR Primer Index 6 (96% over 29bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG4600.11353371210812359No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC4450.10983152584372825No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA4430.10933790100847553Illumina PCR Primer Index 6 (96% over 29bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCATGTCCTGGCGGGGAAGCCA4410.10884427617322283No Hit
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA4380.10810383892034375No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA4290.10588252716170657Illumina PCR Primer Index 6 (100% over 28bp)
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4210.10390802782069572No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4170.1029207781501903Illumina PCR Primer Index 6 (96% over 29bp)
ATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGC4070.10045265397392673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)