FastQCFastQC Report
Thu 22 Jun 2023
ERR2382590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288711
Total Bases306.9 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA68449329.907358334014212Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2027798.859965281767773Illumina PCR Primer Index 6 (96% over 29bp)
ATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1581056.90803688189553Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC1155235.047513644142926No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1025454.4804695743586676Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA879983.84487163298468Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA703983.0758798293012966Illumina PCR Primer Index 6 (96% over 29bp)
AAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT384421.6796353930225356Illumina PCR Primer Index 6 (96% over 27bp)
TAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT334671.4622641303336246Illumina PCR Primer Index 6 (96% over 27bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA209260.9143137774930955Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT171630.7498980867396539Illumina PCR Primer Index 6 (96% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA159580.6972483638170132Illumina PCR Primer Index 6 (100% over 28bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA151140.6603717114131055Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA137360.600163148601986Illumina PCR Primer Index 6 (96% over 29bp)
TGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCC117470.51325833624254No Hit
AGGTGGGGAGTTTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC104710.4575064304754947Illumina PCR Primer Index 6 (100% over 22bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC99170.4333006657459155Illumina PCR Primer Index 6 (96% over 29bp)
GTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC90810.39677355507095485No Hit
CTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC89510.39109350197556614No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA78870.3446044520256162RNA PCR Primer, Index 37 (96% over 27bp)
TAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC76060.332326798796353Illumina PCR Primer Index 6 (95% over 23bp)
GAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT75790.33114709546115695Illumina PCR Primer Index 6 (96% over 27bp)
AAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACCAT73270.3201365309993267No Hit
CAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT62490.27303578302371945Illumina PCR Primer Index 6 (96% over 27bp)
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC61460.2685354332635269No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA59760.26110767152340336Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT54580.2384748445740856No Hit
GGGGGAGTTTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC48210.21064258440668132Illumina PCR Primer Index 6 (100% over 25bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA47290.2066228545237909Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA44640.19504428475242178Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC44240.19329657610768683No Hit
TAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA43330.18932053894091477Illumina PCR Primer Index 6 (96% over 29bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA39890.1742902445961941No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC37590.16424091988896805No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA37570.1641535344567313Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA37330.16310490926989035Illumina PCR Primer Index 6 (100% over 28bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATAGCTCGTATGC36670.16022119000607765No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA36070.1575996270389752Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA34360.15012817258273325TruSeq Adapter, Index 6 (96% over 29bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACCAT34020.14864262023470853No Hit
ATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCG31750.13872437367583762No Hit
ATTACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA31260.13658343058603728TruSeq Adapter, Index 6 (96% over 29bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA30690.13409294576728997Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA29880.13055383576170168Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA29360.1282818145235462Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGACCATCAATATCTCGTATGCC29010.12675256945940314No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA28670.12526701711137841Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA27410.11976173488046328RNA PCR Primer, Index 37 (96% over 27bp)
TAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGT23050.1007117106528522No Hit
ATAGCTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAA23030.10062432522061544TruSeq Adapter, Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)