FastQCFastQC Report
Thu 22 Jun 2023
ERR2382592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA169432842.358200000000004Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48549012.13725Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA43221710.805425RNA PCR Primer, Index 37 (96% over 27bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2045995.114975Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA1233723.0843Illumina PCR Primer Index 6 (96% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1010502.52625Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA515681.2892000000000001RNA PCR Primer, Index 37 (96% over 27bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA450291.125725Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC441091.102725No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA360660.9016500000000001Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA310500.77625Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA286480.7162Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA254320.6358Illumina PCR Primer Index 6 (96% over 28bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA222380.55595Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA195720.48929999999999996Illumina PCR Primer Index 6 (100% over 28bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA156410.39102499999999996Illumina PCR Primer Index 6 (100% over 28bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA143770.359425Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA124680.3117Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCNTATGCCGTCTTCTGCTTGAAAAAAAAAAA111310.278275RNA PCR Primer, Index 37 (96% over 27bp)
CCTGCGGAAGGATCATTACTNTAGGCACCATCAATATCTCGTATGCCGTC109500.27375No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA105880.2647Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA101270.25317500000000004Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA90580.22645RNA PCR Primer, Index 37 (96% over 27bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA85020.21255Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA77580.19395Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA77540.19385Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATNTCTCGTATGCCGTCTTCTGCTTGAAAAAA76950.192375TruSeq Adapter, Index 6 (96% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA75370.188425Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA74490.186225Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAANATCTCGTATGCCGTCTTCTGCTTGAAAAA72760.1819TruSeq Adapter, Index 6 (96% over 28bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA72320.1808Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA69370.173425Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA57670.144175Illumina Single End Adapter 1 (95% over 22bp)
TCGTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA56740.14185RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA54570.136425RNA PCR Primer, Index 44 (100% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT51110.127775No Hit
TAGGCACCATCAATATCTCGNATGCCGTCTTCTGCTTGAAAAAAAAAAAA50050.125125Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACAATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA42650.106625Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA40360.10089999999999999RNA PCR Primer, Index 44 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)