FastQCFastQC Report
Thu 22 Jun 2023
ERR2382593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218646
Total Bases29.5 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA11457252.40068421100775RNA PCR Primer, Index 37 (96% over 27bp)
GCTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA3418815.636233912351472Illumina PCR Primer Index 6 (96% over 28bp)
AGCTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA143806.576841103884819RNA PCR Primer, Index 37 (96% over 27bp)
TGTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA70983.2463434044071238Illumina PCR Primer Index 6 (96% over 28bp)
GTAGGCACCATCAATATCTCNTATGCCGTCTTCTGCTTGAAAAAAAAAAA30161.3793986626784849RNA PCR Primer, Index 37 (96% over 27bp)
CCTGCGGAAGGATCATTACTNTAGGCACCATCAATATCTCGTATGCCGTC28771.3158255810762602No Hit
GTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA25171.1511758733294915RNA PCR Primer, Index 37 (96% over 27bp)
AAGCTGTAGGCACCATCAATNTCTCGTATGCCGTCTTCTGCTTGAAAAAA21620.9888129670792056TruSeq Adapter, Index 6 (96% over 28bp)
CAAGCTGTAGGCACCATCAANATCTCGTATGCCGTCTTCTGCTTGAAAAA19270.8813332967445094TruSeq Adapter, Index 6 (96% over 28bp)
TCGTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA15720.7189703904942235RNA PCR Primer, Index 37 (96% over 27bp)
TAGGCACCATCAATATCTCGNATGCCGTCTTCTGCTTGAAAAAAAAAAAA12390.5666694108284624Illumina PCR Primer Index 6 (96% over 28bp)
CGTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA10970.501724248328348Illumina PCR Primer Index 6 (96% over 28bp)
TTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA9990.4569029389972833RNA PCR Primer, Index 37 (96% over 27bp)
GCGTCGTGTAGGCACCATCANTATCTCGTATGCCGTCTTCTGCTTGAAAA8820.4033917839795834RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA6820.31191972412026747RNA PCR Primer, Index 37 (96% over 27bp)
GTCGTGTAGGCACCATCAATNTCTCGTATGCCGTCTTCTGCTTGAAAAAA6120.2799045031695069TruSeq Adapter, Index 6 (96% over 28bp)
ACTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA5430.24834664251804286RNA PCR Primer, Index 37 (96% over 27bp)
AGGCACCATCAATATCTCGTNTGCCGTCTTCTGCTTGAAAAAAAAAAAAA5380.24605984102155998RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCATCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA5240.23965679683140784RNA PCR Primer, Index 37 (96% over 27bp)
CGTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA5220.23874207623281468RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACTATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA5150.2355405541377386Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA4910.22456390695462072RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGTACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA4490.20535477438416433Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACCATAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA3990.18248675941933537RNA PCR Primer, Index 44 (96% over 29bp)
CTGTAGGCACCATCAATATTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA3910.17882787702496272Illumina Single End Adapter 2 (95% over 21bp)
ACCTGCGGAAGGATCATTACNGTAGGCACCATCAATATCTCGTATGCCGT3080.1408669721833466No Hit
CCTGCGGAAGGATCATTACTNTAGGCACCATCAATATCTCGTATGCCTTC3040.13903753098616028No Hit
GTAGGCACCATCAATATCTCNTATGCCGTCTTCTGCTTGAAAAAAAAAAC3040.13903753098616028RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACAATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA2980.1362933691903808Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACCATTAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA2810.12851824410233895Illumina PCR Primer Index 6 (96% over 27bp)
TAGGCACCATCAATATCTCGNATGCCGTCTTCTGCTTGAAAAAAAAAAAC2790.1276035235037458Illumina PCR Primer Index 6 (96% over 28bp)
CTTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA2430.1111385527290689Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACCATCAATATANCGTATGCCGTCTTCTGCTTGAAAAAAAAA2270.10382078794032362Illumina Single End Adapter 2 (95% over 21bp)
CCTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA2200.10061926584524757Illumina PCR Primer Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)