FastQCFastQC Report
Thu 22 Jun 2023
ERR2382596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613614
Total Bases217.8 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA709844.399069418088836RNA PCR Primer, Index 37 (96% over 27bp)
GCTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA205221.2718035416152809Illumina PCR Primer Index 6 (96% over 28bp)
AGCTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA154510.9575400312590249RNA PCR Primer, Index 37 (96% over 27bp)
CAAGCTGTAGGCACCATCAANATCTCGTATGCCGTCTTCTGCTTGAAAAA51090.3166184725715072TruSeq Adapter, Index 6 (96% over 28bp)
AAGCTGTAGGCACCATCAATNTCTCGTATGCCGTCTTCTGCTTGAAAAAA34850.21597482421446515TruSeq Adapter, Index 6 (96% over 28bp)
TGTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA24700.1530725439913139Illumina PCR Primer Index 6 (96% over 28bp)
GCGTCGTGTAGGCACCATCANTATCTCGTATGCCGTCTTCTGCTTGAAAA16830.10430003705966853RNA PCR Primer, Index 37 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)