FastQCFastQC Report
Fri 23 Jun 2023
ERR2382597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3579058.947624999999999No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT1656014.140025No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1558443.8961Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1455143.6378500000000003No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1396803.492Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1014392.535975Illumina PCR Primer Index 6 (96% over 29bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT900802.252No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA882882.2072000000000003No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA876442.1911No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA716601.7915Illumina PCR Primer Index 6 (100% over 28bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT526331.315825No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC491351.228375No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT466441.1661No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA463761.1594Illumina PCR Primer Index 6 (96% over 29bp)
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT409911.024775No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC345440.8635999999999999No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA330250.8256249999999999No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA253930.634825No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT245420.61355No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA227360.5684No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT212820.53205No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA212180.53045No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA205590.5139750000000001No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA202170.505425No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC198110.49527499999999997No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT194570.48642499999999994No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATAT190800.477No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA183280.4582No Hit
GTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA156690.391725No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT153590.383975No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT151650.379125No Hit
CTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA141910.354775No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATC136900.34225No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA133790.334475Illumina PCR Primer Index 6 (100% over 28bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA128210.320525No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT127340.31835No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT126300.31575000000000003No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT124870.312175No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT120040.30010000000000003No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA118690.29672499999999996No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC118520.2963No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA117500.29375No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT107710.269275No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT105260.26315No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC104360.2609No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCAA98190.245475No Hit
TTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT93450.23362499999999997No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA85590.21397500000000003No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA82150.205375No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT81740.20435000000000003No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC78910.197275No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA78660.19665No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG76270.190675No Hit
ATTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA75370.188425No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA69700.17425No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATCT68920.17229999999999998No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC67110.167775No Hit
GTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA65680.1642No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT65020.16255No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA64120.1603No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA63830.159575Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA62400.156Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT59710.149275No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA57230.143075No Hit
TAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT56510.14127499999999998No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC55510.138775No Hit
TTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAA53480.1337No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA53120.1328Illumina PCR Primer Index 6 (100% over 28bp)
TTGTGGCGTCGCTGAACCATAGCAGGCTACTGTAGGCACCATCAATATCT52610.131525No Hit
CTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA52600.1315No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT47280.11820000000000001No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT43660.10915No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT43090.10772500000000002No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATC42800.107No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT42080.1052No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT41300.10325No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC41070.10267500000000002No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT40590.10147500000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)