FastQCFastQC Report
Fri 23 Jun 2023
ERR2382599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3548018.870025No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT1655974.139925No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1555493.888725Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1445993.6149750000000003No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1400683.5017Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1016372.540925Illumina PCR Primer Index 6 (96% over 29bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT897172.242925No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA878632.1965749999999997No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA871172.177925No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA720451.8011249999999999Illumina PCR Primer Index 6 (100% over 28bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT521821.3045499999999999No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC485501.21375No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA463731.159325Illumina PCR Primer Index 6 (96% over 29bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT462281.1557No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT405121.0128No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC348040.8701000000000001No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA328790.821975No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA253200.633No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT246420.61605No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA223680.5591999999999999No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA212250.530625No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT211700.52925No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA203540.50885No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC201500.50375No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA200950.5023749999999999No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT194890.48722499999999996No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATAT185300.46325000000000005No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA179400.4485No Hit
GTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA153150.382875No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT152220.38055No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT150640.3766No Hit
CTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA141390.35347500000000004No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATC135130.33782500000000004No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA132280.3307Illumina PCR Primer Index 6 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT127710.319275No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA126310.315775No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT124690.311725No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT123490.308725No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT120740.30185No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA118160.2954No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC116970.29242500000000005No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA115990.289975No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT105350.26337499999999997No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC104840.2621No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT103590.258975No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCAA97610.24402500000000002No Hit
TTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT92670.231675No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA82210.20552499999999999No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA81520.20379999999999998No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT78850.197125No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA78170.195425No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG76950.192375No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC76290.190725No Hit
ATTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA74350.18587499999999998No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA68980.17245No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATCT68780.17195000000000002No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC64920.1623No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA64880.16219999999999998No Hit
GTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA64520.1613No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT63660.15915No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA63480.1587Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA62280.1557Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT59940.14985No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA57510.14377500000000001No Hit
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC56660.14165No Hit
TAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT55840.1396No Hit
TTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAA54400.136No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTACTGTAGGCACCATCAATATCT52650.131625No Hit
CTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA52130.130325No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA50020.12505000000000002Illumina PCR Primer Index 6 (100% over 28bp)
GTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT46980.11745No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT43250.108125No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT43200.108No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATC41900.10475000000000001No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT41230.103075No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT41150.102875No Hit
ACCTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATC40940.10235000000000001No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC40780.10195No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATC40650.10162500000000001No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT40260.10065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)