FastQCFastQC Report
Fri 23 Jun 2023
ERR2382600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3450878.627175No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT1640904.102250000000001No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1547083.8677Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1419673.5491750000000004No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1397513.493775Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1063272.658175Illumina PCR Primer Index 6 (96% over 29bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT891932.229825No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA870322.1757999999999997No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA854992.1374750000000002No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA747741.8693499999999998Illumina PCR Primer Index 6 (100% over 28bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT515681.2892000000000001No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC475681.1892No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA454871.137175Illumina PCR Primer Index 6 (96% over 29bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT452801.1320000000000001No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT402511.006275No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC343100.85775No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA323020.80755No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA253970.634925No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT243610.609025No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA227140.5678500000000001No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA213710.534275No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT204670.511675No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA202760.5069No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA200370.500925No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC193340.48335No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT192180.48045No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATAT185500.46375No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA179920.44980000000000003No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT151140.37785No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT150680.3767No Hit
GTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA150410.376025No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA138330.345825Illumina PCR Primer Index 6 (100% over 28bp)
CTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA137190.34297500000000003No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATC128640.3216No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA125000.3125No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT124770.311925No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT123660.30915No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT121850.304625No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT120040.30010000000000003No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA119930.299825No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC116130.290325No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA113320.2833No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT107270.268175No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT106570.266425No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC101810.254525No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCAA95200.23800000000000002No Hit
TTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT89190.222975No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA84470.211175No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA81850.204625No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA79400.19849999999999998No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT78690.19672499999999998No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC76590.191475No Hit
ATTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA74180.18545No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG72390.180975No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA71150.177875No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC66030.165075No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATCT64950.162375No Hit
GTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA64790.161975No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT63990.159975No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA61950.154875No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA59020.14755000000000001Illumina PCR Primer Index 6 (96% over 29bp)
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT58990.147475No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA57410.14352499999999999No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA56540.14135Illumina PCR Primer Index 6 (96% over 29bp)
TTCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATC56100.14025No Hit
TAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT56050.140125No Hit
TTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAA52700.13175No Hit
CTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA51050.12762500000000002No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTACTGTAGGCACCATCAATATCT48900.12225000000000001No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT46030.11507500000000001No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT44680.1117No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT42970.10742499999999999No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT42500.10625000000000001No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA42360.10590000000000001Illumina PCR Primer Index 6 (100% over 28bp)
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC41820.10455No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT41280.10319999999999999No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT41010.10252499999999999No Hit
ACCTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATC40290.100725No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATC40120.1003No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATC40040.1001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)