FastQCFastQC Report
Fri 23 Jun 2023
ERR2382603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA2392765.9818999999999996No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1105772.7644249999999997No Hit
AAATACTGCCAGCTGGGACTNAGGACTGCGACGCTGTAGGCACCATCAAT1104972.762425No Hit
CAAGCTGTAGGCACCATCAANATCTCGTATGCCGTCTTCTGCTTGAAAAA1037762.5944TruSeq Adapter, Index 6 (96% over 28bp)
TTTGCTCGAATATATTAGCANGGAATAATAGAATCTGTAGGCACCATCAA968762.4219No Hit
CTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA933282.3331999999999997RNA PCR Primer, Index 37 (96% over 27bp)
AGCTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA665541.66385RNA PCR Primer, Index 37 (96% over 27bp)
TATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT597211.493025No Hit
ATTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA588801.472No Hit
TTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA585211.463025No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT533321.3333No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA484501.21125Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA475731.189325Illumina PCR Primer Index 6 (96% over 28bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA456411.141025No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA447561.1189Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA350590.876475Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTCGAATATATTAGCANGGAATAATAGAACTGTAGGCACCATCAAT348520.8713000000000001No Hit
AAGCTGTAGGCACCATCAATNTCTCGTATGCCGTCTTCTGCTTGAAAAAA306120.7653TruSeq Adapter, Index 6 (96% over 28bp)
TTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT305570.763925No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT288910.722275No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA285540.71385No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA275800.6895No Hit
GATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT268550.671375No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA243490.608725Illumina PCR Primer Index 6 (100% over 28bp)
GGGGCCAGCATCAGTTTTGGNGGCAGGATACTGTAGGCACCATCAATATC231790.5794750000000001No Hit
TTTTGCTCGAATATATTAGCNTGGAATAATAGAACTGTAGGCACCATCAA219010.547525No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT164640.41159999999999997No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC154610.386525No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA153530.383825Illumina PCR Primer Index 6 (96% over 29bp)
AATACTGCCAGCTGGGACTGNGGACTGCGACGCTGTAGGCACCATCAATA151590.378975No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT148170.370425No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCCTGTAGGCACCATCAA140550.351375No Hit
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATTTCTGTAGGCACCATCAAT140290.350725No Hit
TGAATACTGCCAGCTGGGACNGAGGACTGCGACGCTGTAGGCACCATCAA136660.34165No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT131640.3291No Hit
CTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT128530.32132499999999997No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC107770.269425No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA105300.26325No Hit
CCAGGGGGCATGCCTGTTTGNGCGTCATTCTGTAGGCACCATCAATATCT100490.25122500000000003No Hit
CTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA96090.240225No Hit
TGTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA85100.21275Illumina PCR Primer Index 6 (96% over 28bp)
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATCTGTAGGCACCATCAATAT85060.21265No Hit
TATTCCAGGGGGCATGCCTGNTTGAGCGTCATTTCTGTAGGCACCATCAA84380.21094999999999997No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA83150.20787499999999998No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT79560.1989No Hit
GGAATACTGCCAGCTGGGACNGAGGACTGCGACGCTGTAGGCACCATCAA78860.19715000000000002No Hit
GTTGCTCGAATATATTAGCANGGAATAATAGAATCTGTAGGCACCATCAA76980.19245No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA73850.184625No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTGTAGGCACCATCAAT70570.176425No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA69120.17279999999999998No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT66920.1673No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA64700.16175No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT64630.161575No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATAAATCCTGTAGGCACCATCAA64550.161375No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA63950.159875No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC62990.157475No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATAT60740.15185No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA58040.1451No Hit
TCCAGGGGGCATGCCTGTTTNAGCGTCATTCTGTAGGCACCATCAATATC50520.1263No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT49760.12440000000000001No Hit
GTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA49730.124325No Hit
ATTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA49680.1242No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT48770.121925No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA44260.11065000000000001Illumina PCR Primer Index 6 (100% over 28bp)
CTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA44130.110325No Hit
GTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA44030.11007499999999999No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA41420.10355No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT41230.103075No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT40890.102225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)