FastQCFastQC Report
Fri 23 Jun 2023
ERR2382604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA3459748.64935No Hit
AAATACTGCCAGCTGGGACTNAGGACTGCGACGCTGTAGGCACCATCAAT1633094.082725No Hit
CAAGCTGTAGGCACCATCAANATCTCGTATGCCGTCTTCTGCTTGAAAAA1537913.8447750000000003TruSeq Adapter, Index 6 (96% over 28bp)
TTTGCTCGAATATATTAGCANGGAATAATAGAATCTGTAGGCACCATCAA1425513.563775No Hit
CTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA1388113.4702749999999996RNA PCR Primer, Index 37 (96% over 27bp)
AGCTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA1038822.59705RNA PCR Primer, Index 37 (96% over 27bp)
TATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT883372.208425No Hit
ATTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA865762.1644No Hit
TTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA848922.1223No Hit
GCTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA737451.843625Illumina PCR Primer Index 6 (96% over 28bp)
TTTGCTCGAATATATTAGCANGGAATAATAGAACTGTAGGCACCATCAAT515771.289425No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATAAATCCACTGTAGGCACCATC485981.21495No Hit
AAGCTGTAGGCACCATCAATNTCTCGTATGCCGTCTTCTGCTTGAAAAAA450081.1252TruSeq Adapter, Index 6 (96% over 28bp)
TTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT448531.121325No Hit
GATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT402241.0056No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATACTGTAGGCACCATCAATATC342950.857375No Hit
TTTTGCTCGAATATATTAGCNTGGAATAATAGAACTGTAGGCACCATCAA315360.7884No Hit
CATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT246520.6163No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTAGCCCTGTAGGCACCATCAA244630.611575No Hit
AATACTGCCAGCTGGGACTGNGGACTGCGACGCTGTAGGCACCATCAATA222190.5554749999999999No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCCTGTAGGCACCATCAA209160.5229No Hit
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATTTCTGTAGGCACCATCAAT206770.5169250000000001No Hit
CTTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA201090.502725No Hit
TGAATACTGCCAGCTGGGACNGAGGACTGCGACGCTGTAGGCACCATCAA197720.4943No Hit
CAGGGGGCATGCCTGTTTGANCGTCATTCTGTAGGCACCATCAATATCTC193990.484975No Hit
CTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT191560.4789No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATAAATCCCTGTAGGCACCATCA182730.45682500000000004No Hit
ATGTACACGGAGTCGAGCACNCGCAACGCGACTGTAGGCACCATCAATAT180890.45222500000000004No Hit
GTTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA154300.38575No Hit
CCAGGGGGCATGCCTGTTTGNGCGTCATTCTGTAGGCACCATCAATATCT152670.381675No Hit
GTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT150340.37585No Hit
CTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA139010.34752500000000003No Hit
TGTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA133230.333075Illumina PCR Primer Index 6 (96% over 28bp)
TTGTGGCGTCGCTGAACCATNGCAGGCTAGCTGTAGGCACCATCAATATC131080.3277No Hit
ATTCCAGGGGGCATGCCTGTNTGAGCGTCATTTCTGTAGGCACCATCAAT125420.31355No Hit
TATTCCAGGGGGCATGCCTGNTTGAGCGTCATTTCTGTAGGCACCATCAA125280.3132No Hit
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATCTGTAGGCACCATCAATAT122750.306875No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTGTAGGCACCATCAAT119440.2986No Hit
GGAATACTGCCAGCTGGGACNGAGGACTGCGACGCTGTAGGCACCATCAA117650.29412499999999997No Hit
AATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT117540.29385No Hit
GTTGCTCGAATATATTAGCANGGAATAATAGAATCTGTAGGCACCATCAA114340.28585No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTCTGTAGGCACCATCAATATC111660.27915No Hit
TCAGGGGGCATGCCTGTTTGNGCGTCATTCTGTAGGCACCATCAATATCT104750.261875No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTGTAGGCACCATCAAT104140.26034999999999997No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTCTGTAGGCACCATCAATATC101860.25465000000000004No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATAAATCCTGTAGGCACCATCAA96180.24045No Hit
TTTTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT89570.22392499999999999No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTCTGTAGGCACCATCA80230.200575No Hit
GGGAATACTGCCAGCTGGGANTGAGGACTGCGACGCTGTAGGCACCATCA79240.1981No Hit
TTTTGATGATTCATAATAACNTTTCGAATCGCATCTGTAGGCACCATCAA77550.193875No Hit
TCCAGGGGGCATGCCTGTTTNAGCGTCATTCTGTAGGCACCATCAATATC75910.189775No Hit
TTTGATGATTCATAATAACTNTTCGAATCGCATCTGTAGGCACCATCAAT75820.18955No Hit
ATTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA74430.186075No Hit
AGGGGGCATGCCTGTTTGAGNGTCATTCTGTAGGCACCATCAATATCTCG73060.18265No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTCTGTAGGCACCATCA68170.170425No Hit
TAGGGGGCATGCCTGTTTGANCGTCATTCTGTAGGCACCATCAATATCTC65580.16394999999999998No Hit
GTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA65410.163525No Hit
TTGCTCGAATATATTAGCATNGAATAATAGAATCTGTAGGCACCATCAAT64710.161775No Hit
TGTGGCGTCGCTGAACCATANCAGGCTAGCTGTAGGCACCATCAATATCT64560.1614No Hit
TTTGATGATTCATAATAACTNTTCGAATCGCACTGTAGGCACCATCAATA61430.153575No Hit
GCAGGGGGCATGCCTGTTTGNGCGTCATTCTGTAGGCACCATCAATATCT60680.1517No Hit
GTAGGCACCATCAATATCTCNTATGCCGTCTTCTGCTTGAAAAAAAAAAA59790.149475RNA PCR Primer, Index 37 (96% over 27bp)
GATTCCAGGGGGCATGCCTGNTTGAGCGTCATTTCTGTAGGCACCATCAA58060.14515No Hit
TTCGAATCTTTGAACGCACANTGCGCCCCTCTGTAGGCACCATCAATATC56810.142025No Hit
CAAGCAGCTGTAGGCACCATNAATATCTCGTATGCCGTCTTCTGCTTGAA56590.141475RNA PCR Primer, Index 6 (96% over 28bp)
TAATACTGCCAGCTGGGACTNAGGACTGCGACGCTGTAGGCACCATCAAT55210.138025No Hit
TTATTCCAGGGGGCATGCCTNTTTGAGCGTCATTCTGTAGGCACCATCAA52610.131525No Hit
CTTTGCTCGAATATATTAGCNTGGAATAATAGAACTGTAGGCACCATCAA51300.12825No Hit
TTGTGGCGTCGCTGAACCATNGCAGGCTACTGTAGGCACCATCAATATCT48300.12075No Hit
TAGGCACCATCAATATCTCGNATGCCGTCTTCTGCTTGAAAAAAAAAAAA46930.117325Illumina PCR Primer Index 6 (96% over 28bp)
GTTGCTCGAATATATTAGCANGGAATAATAGAACTGTAGGCACCATCAAT45870.114675No Hit
GAATACTGCCAGCTGGGACTNAGGACTGCGACGCTGTAGGCACCATCAAT44140.11035No Hit
TGAATGGCTTAGTGAGGCCTNAGGATCTGCTCTGTAGGCACCATCAATAT42670.10667499999999999No Hit
CCCAGGGGGCATGCCTGTTTNAGCGTCATTCTGTAGGCACCATCAATATC42280.1057No Hit
TTTCCAGGGGGCATGCCTGTNTGAGCGTCACTGTAGGCACCATCAATATC41310.103275No Hit
ACCTTGCTCGAATATATTAGNATGGAATAATAGAATCTGTAGGCACCATC40990.10247500000000001No Hit
TTGGGCCAGCATCAGTTTTGNTGGCAGGATACTGTAGGCACCATCAATAT40930.10232500000000001No Hit
TAGTCACTTAGCGATGTACANTGACTGTGCTGTAGGCACCATCAATATCT40440.1011No Hit
AAATATAGATGGATACGAATNAGGCGTCCTGTAGGCACCATCAATATCTC40100.10024999999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)