FastQCFastQC Report
Fri 23 Jun 2023
ERR2382605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485511
Total Bases200.5 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA1267118.529792105208243No Hit
AAATACTGCCAGCTGGGACTNAGGACTGCGACGCTGTAGGCACCATCAAT616704.151433412475573No Hit
CAAGCTGTAGGCACCATCAANATCTCGTATGCCGTCTTCTGCTTGAAAAA560323.7719007129533204TruSeq Adapter, Index 6 (96% over 28bp)
TTTGCTCGAATATATTAGCANGGAATAATAGAATCTGTAGGCACCATCAA529143.562006609173544No Hit
CTGTAGGCACCATCAATATCNCGTATGCCGTCTTCTGCTTGAAAAAAAAA511923.4460869020828526RNA PCR Primer, Index 37 (96% over 27bp)
AGCTGTAGGCACCATCAATANCTCGTATGCCGTCTTCTGCTTGAAAAAAA400042.6929453905087204RNA PCR Primer, Index 37 (96% over 27bp)
TATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT330572.225294864864683No Hit
ATTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA325542.1914344626192603No Hit
TTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA321392.16349794784421No Hit
GCTGTAGGCACCATCAATATNTCGTATGCCGTCTTCTGCTTGAAAAAAAA284461.9148966247977968Illumina PCR Primer Index 6 (96% over 28bp)
TTTGCTCGAATATATTAGCANGGAATAATAGAACTGTAGGCACCATCAAT191841.2914074685411283No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATAAATCCACTGTAGGCACCATC175701.1827579869822573No Hit
AAGCTGTAGGCACCATCAATNTCTCGTATGCCGTCTTCTGCTTGAAAAAA170631.148628317124545TruSeq Adapter, Index 6 (96% over 28bp)
TTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT168161.1320010420656594No Hit
GATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT152601.0272559408849884No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATACTGTAGGCACCATCAATATC129160.8694651200832576No Hit
TTTTGCTCGAATATATTAGCNTGGAATAATAGAACTGTAGGCACCATCAA116910.7870019138195544No Hit
CATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT90830.611439430606707No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTAGCCCTGTAGGCACCATCAA89540.6027555501103661No Hit
AATACTGCCAGCTGGGACTGNGGACTGCGACGCTGTAGGCACCATCAATA86260.5806756059026154No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCCTGTAGGCACCATCAA76620.5157821113408113No Hit
TGAATACTGCCAGCTGGGACNGAGGACTGCGACGCTGTAGGCACCATCAA76590.5155801606315941No Hit
CTTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA75950.5112718788349598No Hit
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATTTCTGTAGGCACCATCAAT75760.509992857676584No Hit
CAGGGGGCATGCCTGTTTGANCGTCATTCTGTAGGCACCATCAATATCTC74340.5004338574403017No Hit
CTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT71350.4803061034216509No Hit
ATGTACACGGAGTCGAGCACNCGCAACGCGACTGTAGGCACCATCAATAT68410.46051493391836207No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATAAATCCCTGTAGGCACCATCA68230.4593032296630587No Hit
GTCCAGGGGGCATGCCTGTTNGAGCGTCATTCTGTAGGCACCATCAATAT57130.3845814672526828No Hit
GTTCCAGGGGGCATGCCTGTNTGAGCGTCATTCTGTAGGCACCATCAATA55540.3738780796641694No Hit
CCAGGGGGCATGCCTGTTTGNGCGTCATTCTGTAGGCACCATCAATATCT55000.37024296689825925No Hit
CTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA52490.3533464242270841No Hit
TGTAGGCACCATCAATATCTNGTATGCCGTCTTCTGCTTGAAAAAAAAAA51530.3468840015321327Illumina PCR Primer Index 6 (96% over 28bp)
ATTCCAGGGGGCATGCCTGTNTGAGCGTCATTTCTGTAGGCACCATCAAT47580.32029382481853047No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTGTAGGCACCATCAAT47000.3163894444403306No Hit
AATTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT46150.3106675076791757No Hit
TATTCCAGGGGGCATGCCTGNTTGAGCGTCATTTCTGTAGGCACCATCAA45480.3061572751733242No Hit
TTGTGGCGTCGCTGAACCATNGCAGGCTAGCTGTAGGCACCATCAATATC45230.30447435259651395No Hit
GGAATACTGCCAGCTGGGACNGAGGACTGCGACGCTGTAGGCACCATCAA45150.30393581737193465No Hit
TTTCCAGGGGGCATGCCTGTNTGAGCGTCATCTGTAGGCACCATCAATAT44960.30265679621355884No Hit
GTTGCTCGAATATATTAGCANGGAATAATAGAATCTGTAGGCACCATCAA43790.2947807185540868No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTCTGTAGGCACCATCAATATC39540.2661710347483122No Hit
TCAGGGGGCATGCCTGTTTGNGCGTCATTCTGTAGGCACCATCAATATCT38970.26233397127318475No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTGTAGGCACCATCAAT38430.2586988585072746No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTCTGTAGGCACCATCAATATC35810.24106182990230296No Hit
GGGGCCAGCATCAGTTTTGGNGGCAGGATAAATCCTGTAGGCACCATCAA35030.23581111146265496No Hit
TTTTCCAGGGGGCATGCCTGNTTGAGCGTCATTCTGTAGGCACCATCAAT32890.221405294205159No Hit
TGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTCTGTAGGCACCATCA30160.2030277796663909No Hit
GGGAATACTGCCAGCTGGGANTGAGGACTGCGACGCTGTAGGCACCATCA30030.20215265992644957No Hit
TTTTGATGATTCATAATAACNTTTCGAATCGCATCTGTAGGCACCATCAA29290.19717120909909114No Hit
TTTGATGATTCATAATAACTNTTCGAATCGCATCTGTAGGCACCATCAAT29110.19595950484378777No Hit
TCCAGGGGGCATGCCTGTTTNAGCGTCATTCTGTAGGCACCATCAATATC27980.18835269479660535No Hit
ATTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA27910.18788147647509848No Hit
AGGGGGCATGCCTGTTTGAGNGTCATTCTGTAGGCACCATCAATATCTCG26450.1780532086265265No Hit
GGTACAACGGGGTATTGTAANCAGTAGAGTAGCCTCTGTAGGCACCATCA25770.17347565921760258No Hit
TTGCTCGAATATATTAGCATNGAATAATAGAATCTGTAGGCACCATCAAT24890.1675517717472304No Hit
GTTTGCTCGAATATATTAGCNTGGAATAATAGAATCTGTAGGCACCATCA24530.16512836323662364No Hit
TAGGGGGCATGCCTGTTTGANCGTCATTCTGTAGGCACCATCAATATCTC24480.16479177872126158No Hit
TGTGGCGTCGCTGAACCATANCAGGCTAGCTGTAGGCACCATCAATATCT22650.1524727854590104No Hit
TTTGATGATTCATAATAACTNTTCGAATCGCACTGTAGGCACCATCAATA22510.15153034881599664No Hit
GCAGGGGGCATGCCTGTTTGNGCGTCATTCTGTAGGCACCATCAATATCT22310.15018401075454843No Hit
GTAGGCACCATCAATATCTCNTATGCCGTCTTCTGCTTGAAAAAAAAAAA21790.14668353179478308RNA PCR Primer, Index 37 (96% over 27bp)
TTCGAATCTTTGAACGCACANTGCGCCCCTCTGTAGGCACCATCAATATC21250.1430484190288729No Hit
GATTCCAGGGGGCATGCCTGNTTGAGCGTCATTTCTGTAGGCACCATCAA21220.14284646831965567No Hit
TAATACTGCCAGCTGGGACTNAGGACTGCGACGCTGTAGGCACCATCAAT20470.13779770058922486No Hit
CAAGCAGCTGTAGGCACCATNAATATCTCGTATGCCGTCTTCTGCTTGAA19100.12857528486830458RNA PCR Primer, Index 6 (96% over 28bp)
TTATTCCAGGGGGCATGCCTNTTTGAGCGTCATTCTGTAGGCACCATCAA18870.12702699609763912No Hit
CTTTGCTCGAATATATTAGCNTGGAATAATAGAACTGTAGGCACCATCAA18660.1256133411331185No Hit
GTTGCTCGAATATATTAGCANGGAATAATAGAACTGTAGGCACCATCAAT17320.11659287612141546No Hit
TTGTGGCGTCGCTGAACCATNGCAGGCTACTGTAGGCACCATCAATATCT17240.11605434089683618No Hit
TAGTCACTTAGCGATGTACANTGACTGTGCTGTAGGCACCATCAATATCT15890.10696655898206071No Hit
TGAATGGCTTAGTGAGGCCTNAGGATCTGCTCTGTAGGCACCATCAATAT15840.10662997446669867No Hit
GAATACTGCCAGCTGGGACTNAGGACTGCGACGCTGTAGGCACCATCAAT15720.10582217162982974No Hit
CCCAGGGGGCATGCCTGTTTNAGCGTCATTCTGTAGGCACCATCAATATC15430.10386998144072983No Hit
ACCTTGCTCGAATATATTAGNATGGAATAATAGAATCTGTAGGCACCATC15300.10299486170078848No Hit
AAATATAGATGGATACGAATNAGGCGTCCTGTAGGCACCATCAATATCTC15280.10286022789464366No Hit
CCAGGGGGCATGCCTGTTTGNGCGTCATTTCTGTAGGCACCATCAATATC15220.10245632647620921No Hit
TTTTGATGATTCATAATAACNTTTCGAATCGCACTGTAGGCACCATCAAT15220.10245632647620921No Hit
TTGGGCCAGCATCAGTTTTGNTGGCAGGATACTGTAGGCACCATCAATAT15030.10117730531783341No Hit
TTTCCAGGGGGCATGCCTGTNTGAGCGTCACTGTAGGCACCATCAATATC14980.10084072080247133No Hit
TAGGCACCATCAATATCTCGNATGCCGTCTTCTGCTTGAAAAAAAAAAAA14970.10077340389939891Illumina PCR Primer Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)