FastQCFastQC Report
Fri 23 Jun 2023
ERR2382606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373459
Total Bases50 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA341169.135139332563948Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA89932.408028726044894No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG87612.3459067795929407No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC86662.320468913588908No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT85392.286462503246675No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC77912.0861727793412395TruSeq Adapter, Index 6 (96% over 26bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA66731.7868092615253615No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA52701.4111321456973858No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC46051.2330670836691577No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA44761.1985251393057874Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG44581.1937053331155494No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC42281.1321189206847337No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC42031.1254247454205148No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA39971.0702647412433492Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC36310.9722620153751818No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT35400.9478952174134242Illumina PCR Primer Index 6 (95% over 21bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG35030.93798783802238No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC32410.8678328812533639No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA31740.8498924915452566Illumina PCR Primer Index 6 (100% over 28bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC30270.8105307409916483No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC30180.8081208378965294No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC28320.7583161739307394No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG23830.6380887861853644No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG22670.6070278129593878No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA22260.5960493655260685No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG21240.5687371304480545No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCCTG18710.5009920767741572No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC18620.49858217367903834No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC18290.4897458623302692No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG18080.4841227551083251No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG17120.45841712209372376No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16900.45252624786121104Illumina PCR Primer Index 6 (96% over 29bp)
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC15150.4056670210116773No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA14590.39067206841982655Illumina PCR Primer Index 6 (96% over 29bp)
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC14260.3818357570710573No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC14250.3815679900604886No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC14160.3791580869653697No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA13800.3695184745848942Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC13750.36817963953205035No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG13680.36630527045806904No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC13520.36202099828896883No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC13440.35987886220441867No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG13390.35854002715157485No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA13140.3518458518873558No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCC12930.34622274466541175No Hit
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT12750.341402938475174Illumina PCR Primer Index 6 (95% over 21bp)
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC12130.32480138381991064No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT11110.2974891487418967No Hit
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10670.2857074002768711No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCC10400.2784776909915145No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA10270.27499671985412055No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10230.27392565181184547Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA10000.26776701056876395Illumina PCR Primer Index 6 (96% over 29bp)
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT9990.26749924355819515RNA PCR Primer, Index 6 (95% over 22bp)
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9950.2664281755159201No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9620.2575918641671509No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCCT9560.2559852621037383No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG9200.2463456497232628No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT9050.24232914456473134No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA9020.24152584353302506No Hit
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG9000.24099030951188752No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC8890.2380448723956311No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8850.23697380435335605No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCCA8820.23617050332164977No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT8670.2321539981631183No Hit
TCCTGAGTGCGAGCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT8670.2321539981631183Illumina PCR Primer Index 6 (95% over 21bp)
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA8660.23188623115254955No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG8410.22519205588833044No Hit
GTCCTGGCGGGGAAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT8350.22358545382491785Illumina PCR Primer Index 6 (95% over 21bp)
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8270.22144331774036777No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA7810.20912603525420462No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA7350.1968087527680415Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT7170.19198894657780372Illumina PCR Primer Index 6 (95% over 21bp)
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCACTGTAGGCACC7160.19172117956723497No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC6810.1823493341973282No Hit
GTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCCCCAGTTCGT6700.17940389708107182No Hit
GGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCTT6660.17833282903879677No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC6490.1737807898591278No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC6460.1729774888274215No Hit
TCCTGGGCGAAATCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT6460.1729774888274215Illumina PCR Primer Index 6 (95% over 21bp)
TCTGACTTCTAATCAGAAGATTATGGGTCCCCCCCATCGTGAGTGCCACT6190.16574777954206485No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5950.1593213712884145Illumina PCR Primer Index 6 (95% over 21bp)
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA5880.1574470022144332No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCA5820.1558404001510206No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCA5610.15021729292907657No Hit
GCGGCGCTACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT5350.14325535065428868Illumina PCR Primer Index 6 (96% over 27bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGTGCCAGATCGGGG5270.14111321456973858No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG5270.14111321456973858No Hit
TGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG5160.13816777745348216No Hit
GCGGCGCTACCACTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGC5100.1365611753900696Illumina PCR Primer Index 6 (96% over 26bp)
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC4960.1328124372421069No Hit
TATGACTCTTAATCATAATGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA4880.1306703011575568No Hit
ATGCCTTGCTAAGGCATTGGGTTTTACCTGCGCAGGTTCGAATCCTGTCT4800.12852816507300668No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACCA4710.1261182619778878No Hit
TCTGACTTCTAATCAGAATATTATGGGTTCCCCCCATCGTGAGTGCCACT4620.12370835888276892No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCTGT4590.12290505785106264No Hit
TCCACAGAATTCGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT4550.12183398980878757Illumina PCR Primer Index 6 (95% over 21bp)
TCCTGGGCGAAATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT4540.12156622279821883Illumina PCR Primer Index 6 (95% over 24bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCC4460.11942408671366869No Hit
TTGCATTTGTAATGCAAAGGTCGTTAGTTCATTTCTGACAGGTGGCACCA4420.11835301867139365No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4390.11754971763968736No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC4280.11460428052343094No Hit
GCAGACTTAAGATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCA4130.11058777536489948No Hit
TCAGTCTCATAATCTGAAGGTCGAGAGTTCGAACCTCCCCTGTAGGCACC4080.10924894031205568No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAAGTCCTGGCGGGGAAGCCA3750.10041262896328647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)