FastQCFastQC Report
Fri 23 Jun 2023
ERR2382610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380438
Total Bases56.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA13221634.7536260836194Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA304468.002880889921617Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA279487.346269300122491Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA255966.728034528622271Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA143923.783008006560859Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA99402.6127779033640173Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC77632.0405427428385177TruSeq Adapter, Index 6 (96% over 26bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA55961.4709361315115734Illumina PCR Primer Index 6 (100% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48401.272217812100789Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC34090.8960724217875187No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA32990.8671583806034098Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA28220.7417765838323196Illumina PCR Primer Index 6 (96% over 29bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG20700.5441096841009573No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA20360.5351726168258691No Hit
ACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19030.5002129124850829Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA18910.4970586534468166Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC18540.4873330214121617No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC17280.4542133015103643No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC16650.4376534415594656No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15960.4195164520894338Illumina PCR Primer Index 6 (96% over 29bp)
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG15900.4179393225703006No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC15460.406373706096657No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG14830.3898138461457583No Hit
GACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA14820.38955099122590275Illumina PCR Primer Index 6 (96% over 29bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC13900.36536833859919354No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT13830.36352835416020485No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA11450.3009688832345875No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG10510.2762605207681672No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC10490.27573481092845614No Hit
CTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9910.2604892255768351Illumina PCR Primer Index 6 (100% over 28bp)
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC9680.2544435624201578No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA9170.24103796150752554Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8550.22474095647648235No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7840.20607825716673936No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC7790.20476398256746173No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTCTGCCGTCTTCTGC7370.19372407593352925Illumina PCR Primer Index 6 (96% over 25bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG7290.19162123657468497No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC7030.18478700865844105No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC6860.18031847502089696No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA6380.1677014388678313No Hit
CTGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5950.15639867731404328Illumina PCR Primer Index 6 (100% over 28bp)
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA5310.13957596244328904No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC5210.1369474132447337No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC5170.13589599356531157No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC5140.13510742880574494No Hit
TCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA4930.12958747548877872Illumina PCR Primer Index 6 (100% over 28bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4770.12538179677109015No Hit
CTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4620.12143897297325715Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCCTGTAGGCACC4500.11828471393499071No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC4380.11513045489672431No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT4030.10593053270178059RNA PCR Primer, Index 6 (95% over 22bp)
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC4020.10566767778192505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)