FastQCFastQC Report
Thu 22 Jun 2023
ERR2382612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511573
Total Bases68.5 Mbp
Sequences flagged as poor quality0
Sequence length121-135
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA21166041.374349310851045Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC148522.90320247550203TruSeq Adapter, Index 6 (96% over 26bp)
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC111882.1869801572796064No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC103812.0292314097890234No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA101371.9815353820471369Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC100301.9606195010291785No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA91731.7930969773619794Illumina PCR Primer Index 6 (100% over 28bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA83751.6371075095831875Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA79201.5481661463759817No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA63421.2397057702419791Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT56141.0973995891104495No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC55681.0884077150279627No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA39360.7693916606232151Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA38210.7469119754169982Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA35410.6921788288279483Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA33230.6495651646979023No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC32660.6384230598565601No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC32410.633536171768252No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC32100.6274764305387501No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC30800.6020646124795483No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC28790.5627740322495519No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC27840.5442038575139814No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA26420.5164463331723919Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC24230.47363719351881356No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA23630.4619086621068743Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC23390.4572172495420986No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC22170.43336923567115543No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA21950.42906877415344435No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC20070.39231937572936804No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC19240.37609490727618544No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC17000.3323083900049455No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC16880.3299626837225577No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC16110.314911068410569No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC15870.3102196558457933No Hit
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC14160.27679334132176636No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA14150.27659786579823403Illumina PCR Primer Index 6 (96% over 29bp)
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC13480.2635010057215686No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC13130.25665936239793735Illumina PCR Primer Index 6 (96% over 29bp)
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC13090.25587746030380804No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG11590.22655613177395992No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC11320.22127829263858725No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10980.21463212483848834Illumina PCR Primer Index 6 (96% over 29bp)
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG10970.214436649314956No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC10490.2050538241854046No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10440.20407644656774301No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10110.19762575429117646RNA PCR Primer, Index 37 (96% over 27bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA8550.16713157262013437Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT8360.16341753767302028Illumina PCR Primer Index 6 (95% over 24bp)
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8340.16302658662595565No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA7720.15090710416695172TruSeq Adapter, Index 6 (96% over 29bp)
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC7510.146802118172773No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC7170.1401559503726741No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7020.1372238175196893Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA6470.12647266372541163RNA PCR Primer, Index 37 (96% over 27bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6390.12490885953715306Illumina PCR Primer Index 6 (100% over 28bp)
TCCACAGAGTTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC6270.1225631532547652No Hit
TCCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC5820.11376675469581077No Hit
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA5780.11298485260168149Illumina PCR Primer Index 6 (96% over 29bp)
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA5770.11278937707814916No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5710.11161652393695523Illumina PCR Primer Index 6 (96% over 29bp)
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5680.11103009736635827No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC5680.11103009736635827No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA5480.10712058689571186No Hit
TCAGTCTCATAATCTGAAGGTCGAGAGTTCGAACCTCCCCTGTAGGCACC5270.10301560090153312No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT5270.10301560090153312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)