FastQCFastQC Report
Fri 23 Jun 2023
ERR2382613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4261724
Total Bases571.4 Mbp
Sequences flagged as poor quality0
Sequence length121-135
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA187040343.88841229511812Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT1870564.389209625024989No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC1603313.7621159887407067No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC1491723.5002735981964106No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA877282.058509654778207Illumina PCR Primer Index 6 (100% over 28bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT686581.6110381620208158No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA625171.4669415475990468Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC560351.314843476489796TruSeq Adapter, Index 6 (96% over 26bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA552811.2971511059843388Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA542421.2727713010040067Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA541671.2710114498264082Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA527451.2376446714991398Illumina PCR Primer Index 6 (100% over 28bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA434661.019915883806647No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC297400.6978396536237447No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC288350.6766041160807224No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC282650.6632292471309733No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC233620.5481819094807642No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC177910.41746016400874386No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC171500.4024193026108683No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC152150.3570151422288257No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA150560.3532842577323168Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA148070.3474415518226896Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA117840.27650781702428406Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT114170.2678962785952352No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC103340.2424840275907121Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA97990.22993042252384246Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA93220.21873776903431572RNA PCR Primer, Index 37 (96% over 27bp)
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT92990.21819808133985213No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA91750.2152884607262225No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA87820.20606684055560612Illumina PCR Primer Index 6 (96% over 29bp)
TGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCC85030.19952019417493952No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC78360.18386925103549642No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC76210.1788243443263806No Hit
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA68350.1603811039851478RNA PCR Primer, Index 37 (96% over 27bp)
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC67230.15775305955993396No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA64560.15148798936768312Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTGT61350.14395582632756135No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA60880.14285298625626625Illumina PCR Primer Index 6 (96% over 29bp)
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC60570.14212558110285883No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA57510.13494538829825675Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA57460.13482806488641685No Hit
ACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTC54270.12734283121103104No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC52610.12344769393794625No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC52290.12269682410217086No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC51290.1203503558653728No Hit
CTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC49280.11563395470940868No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC49240.11554009597993675No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATAGCTCGTATGC47690.11190307021289976No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG47550.11157456465974802No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG46470.10904037896400612No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC45280.10624808176221642No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA45140.10591957620906468Illumina PCR Primer Index 6 (100% over 28bp)
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC44120.10352617860753066No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA43950.10312727900727497Illumina PCR Primer Index 6 (96% over 29bp)
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG42810.1004523052173252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)