FastQCFastQC Report
Fri 23 Jun 2023
ERR2382618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629908
Total Bases84.5 Mbp
Sequences flagged as poor quality0
Sequence length121-135
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA449597.1373914920909085Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA222343.5297218006439035Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC216263.4331997688551343TruSeq Adapter, Index 6 (96% over 26bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA181462.8807381395378373Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC139172.2093702572439153No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA136662.169523168462696Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA121751.9328219359017507Illumina PCR Primer Index 6 (96% over 29bp)
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC104581.6602424481035327No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG101341.6088063653739912No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG92001.4605307441721649No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA78591.247642512874896No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC78031.2387523257364568No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT73121.1608044349333553No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC72201.1461991274916337No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC70181.1141309524565493No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG62480.9918908793030093No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC59130.9387085098141316No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA57520.9131492217911187No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC54540.8658407259472811No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC50460.8010693625100809No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC48240.7658261206398395No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC41640.6610489150796625No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG39360.624853153158874No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG36820.5845298043523817No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC36620.5813547375172247No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC33770.5361100351162392No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG32590.5173771407888136No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGGGCCCCCTACAGGGCTCCA32380.5140433206118988No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30120.4781650653746261Illumina PCR Primer Index 6 (100% over 28bp)
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA29260.4645122779834515No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG23390.3713240663715971No Hit
ATCTGGAGGTCCTGTGTTCGGTCCACAGAATTCGCACCACTGTAGGCACC23210.36846650621995597No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT22350.3548137188287814No Hit
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG22150.35163865199362443No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACCA22040.3498923652342882No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCATGTCCTGGCGGGGAAGCCA21280.33782711126069204No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC20660.3279844040717057No Hit
CTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCA19780.31401410999701546No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCCA19620.31147405652888993No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC19590.3109977965036164No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGTGCCCCCTACAGGGCTCCA18100.28734354858169764No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT17570.2789296214685319No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC17520.2781358547597427No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAGCCCTCACTGGGGTCGCCA16440.26099049384989553No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC16340.2594029604323171No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGCTCCACT15410.24463889964883762No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA14830.23543120582688268Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC14730.23384367240930423No Hit
GCCCCCTACAGGGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC13900.22066714504340315No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC13750.2182858449170355No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC13470.21384075134781586No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA13430.21320573798078452No Hit
GGGGCGTGTGGTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCT13280.21082443785441682No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCCTGTAGGCACCA13100.20796687770277567No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAGTTCGCACCACTGTAGGCACC12180.193361570261054No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12120.19240905021050694Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGCTCCA11980.1901865034258971No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA11900.18891647669183437Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC11470.18209008299624707No Hit
GTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG11310.17955002952812157No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAAGTCCTGGCGGGGAAGCCA11180.1774862360852696No Hit
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10920.17335864919956565No Hit
TGGGAGAGGTCTCCGGTTCGTTTCCGGACTCGTCCACCACTGTAGGCACC10770.170977349073198No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC10670.16938981565561953No Hit
TATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCAC10600.16827854226331462No Hit
GTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG10570.1678022822380411No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10410.16526222876991561Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10400.16510347542815776RNA PCR Primer, Index 22 (96% over 27bp)
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10160.16129339522596953No Hit
TATGACCCTTAATCATAAGGTTAGGGGTTCGGGCCCCCTACAGGGCTCCA10130.16081713520069596No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC9840.1562132882897185No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA9780.15526076823917145No Hit
ATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA9730.15446700153038223Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA9570.1519269480622567No Hit
ACCTCCCCTGGAGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT8940.14192548753151252Illumina PCR Primer Index 6 (95% over 21bp)
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTTTTAACCGAAATGTCAG8920.14160798084799686No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCA8850.14049670745569195No Hit
CTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG8820.14002044743041842No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA8740.13875042069635565Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA8420.13367031376010466Illumina PCR Primer Index 6 (96% over 29bp)
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGTGCCCCCTATGAGGAGCCA8040.12763768677330659No Hit
TTGCATTTGTAATGCAAAGGTCGCTAGTTCATTTCTGGCAGGTGGCACCA7920.12573264667221246No Hit
ATCCGACTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7870.12493887996342322No Hit
TTATCACATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATC7750.12303383986232909No Hit
GCAGACTTAAGATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCA7610.12081129307771929No Hit
CCTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACC7610.12081129307771929No Hit
GGTTTCGTGGTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCC7490.11890625297662516No Hit
TGGGCGTGTGGTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCT7490.11890625297662516No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG7450.11827123960959378No Hit
CCACATTGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACT7270.1154136794579526No Hit
ACATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGC7190.11414365272388984No Hit
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7160.11366739269861631No Hit
ACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA6940.11017481917994373RNA PCR Primer, Index 6 (96% over 29bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG6930.1100160658381859No Hit
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA6930.1100160658381859No Hit
GTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCCCCAGTTCGT6910.1096985591546702No Hit
ATCACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCA6800.10795227239533392No Hit
TCTGGCTGCGAACCAGGAGATTCCAGGTTCGGGTCCTGGCGGGGAAGCCA6750.1071585056865447No Hit
ACGCTTTCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTG6320.10033211199095741No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)