FastQCFastQC Report
Fri 23 Jun 2023
ERR2382621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561964
Total Bases210.8 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA78295650.12637935317331Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA16647010.657736029767651Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1085576.950032139024971Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC518703.3208191738093835No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA514673.295018323085551Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA270521.731922118563552Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA239211.5314693552476242Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA182491.1683367862511556Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA144040.9221723420001998Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT106750.6834344453521336No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA99610.6377227644171057Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA86500.5537899721120333Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA76180.4877193072311526Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA70490.45129081079973676Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCTTC48700.31178695539717943No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA46990.30083919987912655Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA42570.27254149263363303Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA36040.23073515138633155Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA36010.23054308550004995Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA35310.22606154815347856Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA33310.21325715573470325Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30080.19257806197838107Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA29210.18700815127621379Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA27920.1787493181661037Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA27770.17778898873469554Illumina PCR Primer Index 6 (100% over 28bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG25530.16344806922566718Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAAA25480.16312795941519778Illumina PCR Primer Index 6 (96% over 27bp)
CTGTAGGCACCATCAATATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA22640.1449457221805368Illumina Single End Adapter 1 (95% over 22bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA22570.1444975684458797Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAA22530.14424148059750416Illumina PCR Primer Index 6 (96% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA22210.1421927778105001Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT22080.14136049230327974No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA21320.1364948231841451Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA21070.13489427413179816Illumina PCR Primer Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC20920.13393394470039002No Hit
CTGTAGGCACCATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17940.11485539999641478RNA PCR Primer, Index 44 (100% over 29bp)
AGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA17280.11062995049821892Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACAATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16560.10602036922745979Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)