FastQCFastQC Report
Fri 23 Jun 2023
ERR2382623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3567386
Total Bases478.3 Mbp
Sequences flagged as poor quality0
Sequence length44-135
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA225964563.34175780249179Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2748797.7053338214591856Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA574421.6101986160174424Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA536721.50451899514098Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC423061.185910355649767No Hit
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA416601.1678018582794236RNA PCR Primer, Index 22 (96% over 27bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA275420.7720498987213607Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA231880.6499997477144329Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA218680.6129978645428333Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA203550.570585857543871RNA PCR Primer, Index 40 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA196700.551384122716185Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA168330.4718581056269212Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC129410.3627586137300533Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT125210.3509852872663625No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA94450.26475968678466527Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA83100.23294367360302473Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA82960.2325512293875684Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA76410.21419044645014587Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA71750.2011276604213842Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA63800.17884243532939806Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA63210.17718856327854624RNA PCR Primer, Index 32 (96% over 28bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT60880.1706571702641654No Hit
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA60390.16928361551006815Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA58010.16261206384731006RNA PCR Primer, Index 37 (96% over 27bp)
TGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA57230.1604255889326246Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAACAAAA56670.15885581207079918Illumina PCR Primer Index 6 (96% over 29bp)
AAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT55910.15672540061546467TruSeq Adapter, Index 6 (96% over 27bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA51000.1429618213448166Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA50520.14161629832039482Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA50130.1405230608630521Illumina PCR Primer Index 6 (96% over 29bp)
ATCAACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA49390.1384487128670685TruSeq Adapter, Index 6 (96% over 29bp)
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT47100.13202944677138947No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA45950.12880579785871224Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA43110.12084478663088323Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA41750.11703247139502146Illumina PCR Primer Index 9 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAACAAAAA41290.11574301182995055Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAACAAAAAA37450.10497882763457612Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGACAAAAAAA36420.10209155947800434Illumina PCR Primer Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC36220.10153092488449525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)