FastQCFastQC Report
Thu 22 Jun 2023
ERR2382625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784034
Total Bases137.5 Mbp
Sequences flagged as poor quality0
Sequence length36-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA74962926.926000185342563Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1709166.139149162689823Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA672172.414374249739766Illumina PCR Primer Index 6 (96% over 29bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT661882.3774134942317513No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT436131.5665397764538795No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT425561.5285732861021095No Hit
TCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATATCTCGTATGCC397681.4284308309453118No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA375951.3503786232495725Illumina PCR Primer Index 6 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT295761.0623433478183097No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT258630.9289757237160178No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT256550.921504550590977No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATATCTCGTATG236190.848373259809327No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA231590.8318504730904867No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA217260.7803784005511427Illumina PCR Primer Index 6 (100% over 28bp)
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC214940.7720451689885971No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG178370.6406890145738163No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATGC177290.636809751605045No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA162720.5844756206281964Illumina PCR Primer Index 6 (96% over 29bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACC162250.5827874228547496No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT151640.5446772560967287No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT138180.4963301453933393No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC130900.47018103945569634No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT126920.4558852370337431No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC120480.4327533356273667No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA112120.40272496672095237Illumina PCR Primer Index 6 (96% over 29bp)
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT95650.3435662064471914No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATGC94770.34040532550967406No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT84400.3031572171891579No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA81460.2925970013297251Illumina PCR Primer Index 6 (96% over 29bp)
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG76240.2738472303139976No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATGCC73810.26511888863426236No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC72820.2615628975795554No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAAT71410.2564983042592152No Hit
TTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATATCTCGTATGC71090.25534889300920893No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG67840.2436751850013326No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCAT48550.1743872380868912No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA47900.17205249648531593Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA47000.1688197773446732Illumina PCR Primer Index 6 (96% over 29bp)
TCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTATGC45890.16483275707121392No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT42250.15175820410239244No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT40510.14550828043048325No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGTATGCCG38460.13814486461013048No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA38420.13800118820387972Illumina PCR Primer Index 6 (96% over 29bp)
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCGTATGCCG38130.1369595342585615No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCGTCG37860.1359897185163687Illumina Single End PCR Primer 1 (100% over 33bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA35770.12848262628976514RNA PCR Primer, Index 32 (96% over 28bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATATCT35010.1257527745710002No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTCTGTAGGCACCATCAATATCT34250.12302292285223528No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA33830.12151432058660203Illumina PCR Primer Index 6 (96% over 29bp)
ACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAATAT30720.11034348000060344No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC29760.10689524625058458No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)