FastQCFastQC Report
Fri 23 Jun 2023
ERR2382627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3650869.12715Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1842734.606825Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1642774.106925Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1419063.54765Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA624791.561975Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA185850.464625Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA111450.278625Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA85990.21497500000000003Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA59210.148025Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA57240.1431Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52990.132475Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAAA51010.127525Illumina PCR Primer Index 6 (96% over 27bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA49830.12457499999999999Illumina PCR Primer Index 6 (96% over 29bp)
AGATCGGAAGAGCGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT42940.10735Illumina Single End PCR Primer 1 (95% over 23bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA41720.1043Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)